当前位置: X-MOL 学术Int. J. Food Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Utility of rapid tests to assess the prevalence of indicator organisms (Aerobic plate count, Enterobacteriaceae, coliforms, Escherichia coli, and Listeria spp.) in apple packinghouses
International Journal of Food Microbiology ( IF 5.4 ) Pub Date : 2020-11-04 , DOI: 10.1016/j.ijfoodmicro.2020.108949
Blanca Ruiz-Llacsahuanga , Alexis Hamilton , Robyn Zaches , Ines Hanrahan , Faith Critzer

The 2014 listeriosis outbreak caused by caramel-coated apples was linked to apples cross-contaminated within an apple packing facility. This outbreak has increased the focus on effective cleaning and sanitation methods that must be validated and monitored during apple packing. Thus, rapid and reliable testing methods are necessary for assessing cleanliness in the apple packing industry. The objectives of this study were to assess the prevalence of common indicator organisms [Aerobic plate count (APC), Enterobacteriaceae, coliforms, Escherichia coli, and Listeria spp.] on food contact surfaces (zone 1) in apple packinghouses and to evaluate the utility and accuracy of currently used rapid tests (ATP and glucose/lactose residue swabs). Food contact surfaces were sampled over a 100 cm2 area in five commercial apple packinghouses to evaluate populations of indicator organisms APC, Enterobacteriaceae, coliforms, E. coli (n = 741), and rapid test readings (n = 659). Petrifilm plates were used for the quantification of APC, Enterobacteriaceae, and coliform/E. coli. Rapid tests [ATP swabs (UltraSnap) and glucose/lactose residue swabs (SpotCheck Plus)] were processed on-site. A larger area (0.93 m2) was sampled for the detection of Listeria spp. (n = 747), following a modified protocol of the FDA's Bacteriological Analytical Manual method, and confirmed with PCR and gel electrophoresis via the iap gene. No significant association was found between either rapid test and populations of APC, Enterobacteriaceae, coliforms, E. coli, and Listeria spp. detection. However, recovery of APC (log CFU/100 cm2) was higher with a failed glucose/lactose residue swab surface hygiene result (3.1) than a passed result (2.9) (p = 0.03).

Populations of APC, Enterobacteriaceae, and coliforms were significantly different at each unit operation during the packing process (p ≤ 0.05). This study concluded that ATP and glucose/lactose residue rapid tests were poorly suited for determining microbial load since they were not related to populations of any common indicator organisms or the detection of Listeria spp. These findings emphasize the need to utilize a rapid test, which can be a good indicator of residual matter on a surface, along with traditional microbiological methods to assess cleaning and sanitation practices in apple packinghouses.



中文翻译:

快速测试可用于评估苹果包装厂中指示生物(需氧菌盘计数,肠杆菌科,大肠菌群,大肠杆菌李斯特菌属)的流行

2014年由焦糖涂层苹果引起的李斯特菌病暴发与苹果包装工厂内交叉污染的苹果有关。这次疫情使人们更加关注有效的清洁和卫生方法,这些方法必须在苹果包装过程中得到验证和监控。因此,快速,可靠的测试方法对于评估苹果包装行业的清洁度是必要的。这项研究的目的是评估常见指示生物的流行程度[好氧板数(APC),肠杆菌科,大肠菌,大肠埃希氏菌李斯特菌spp。]放在苹果包装厂的食品接触表面(1区)上,以评估当前使用的快速测试(ATP和葡萄糖/乳糖残留拭子)的实用性和准确性。在五个商业苹果包装厂的100 cm 2区域内取样食物接触表面,以评估指示生物APC,肠杆菌科,大肠菌,大肠杆菌(n = 741)的种群和快速测试读数(n = 659)。Petrifilm板用于定量APC,肠杆菌科和大肠菌/大肠杆菌。快速测试[ATP拭子(UltraSnap)和葡萄糖/乳糖残留拭子(SpotCheck Plus)]在现场进行。更大的面积(0.93 m 2)被采样用于检测李斯特菌属 (n = 747),遵循FDA细菌分析手册方法的改良方案,并通过iap基因进行PCR和凝胶电泳确认。在快速检测与APC,肠杆菌科,大肠菌,大肠杆菌李斯特菌属之间没有显着相关性。检测。但是,葡萄糖/乳糖残留拭子表面卫生检查结果不合格(3.1)时,APC的回收率(log CFU / 100 cm 2)要高于合格结果(2.9)(p = 0.03)。

在包装过程中的每个单元操作中,APC,肠杆菌科和大肠菌群均存在显着差异(p≤0.05)。这项研究得出的结论是,ATP和葡萄糖/乳糖残留快速检测不适用于确定微生物负荷,因为它们与任何常见指示生物的种群或李斯特菌属的检测均无关。这些发现强调需要利用快速测试(可以很好地指示表面残留物)以及传统的微生物学方法来评估苹果包装厂的清洁和卫生习惯,这一点很重要。

更新日期:2020-11-19
down
wechat
bug