Several mosquito-borne viruses (mobovirus) cause infections in Canada. Ecological data on mosquito species and host range in Canada remains elusive. The main aim of the current study is to determine the host range and molecular systematics of mosquito species in Canada. Mosquitoes were collected using BG-Sentinel traps and aspirators at 10 trapping sites in Canada during 2018 and 2019. Mosquitoes collected were identified via morphology and molecular techniques. Mosquito sequences were aligned by MUSCLE algorithm and evolutionary systematics were drawn using MEGA and SDT software. Moreover, the source of blood meals was identified using a DNA barcoding technique. A total of 5,708 female mosquitoes over 34 different taxa were collected. DNA barcodes and evolutionary tree analysis confirmed the identification of mosquito species in Canada. Of the total collected samples, 201 specimens were blood-fed female mosquitoes in 20 different taxa. Four mosquito species represented about half (51.47%) of all collected blood-fed specimens: Aede cinereus (39 specimens, 19.11%), Aedes triseriatus (23, 11.27%), Culex pipiens (22, 10.78%), and Anopheles punctipennis (21, 10.29%). The most common blood meal sources were humans (49 mosquito specimens, 24% of all blood-fed mosquito specimen), pigs (44, 21.5%), American red squirrels (28, 13.7%), white-tailed deers (28, 13.7%), and American crows (16, 7.8%). Here, we present the first analysis of the host-feeding preference of different mosquito species in Canada via molecular techniques. Our results on mosquito distribution and behavior will aid in the development of effective mitigation and control strategies to prevent or reduce human/animal health issues in regards to moboviruses.

几种蚊媒病毒（mobovirus）在加拿大引起感染。关于加拿大蚊子种类和寄主范围的生态数据仍然难以捉摸。本研究的主要目的是确定加拿大蚊子的寄主范围和分子系统。在2018年至2019年期间，使用BG-Sentinel诱捕器和吸气器在加拿大的10个诱捕地点收集了蚊子。收集的蚊子通过形态学和分子技术进行鉴定。通过MUSCLE算法对蚊子序列进行比对，并使用MEGA和SDT软件绘制进化系统。此外，使用DNA条形码技术鉴定了血粉的来源。在34个不同的分类单元中，总共收集了5708只雌性蚊子。DNA条形码和进化树分析证实了加拿大蚊虫种类的鉴定。在收集的所有样本中，有201个标本是用20种不同的类群进行的食血雌性蚊子。四种蚊子约占所有采集的血样的一半（51.47％）：灰纹伊蚊（39个标本，19.11％），三头伊蚊（23，11.27％），淡色库蚊（22，10.78％），以及按蚊（21，10.29％）。最常见的血粉来源是人类（49个蚊子样本，占所有带血蚊子样本的24％），猪（44个，21.5％），美洲红松鼠（28个，13.7％），白尾鹿（28个，13.7个） ％）和美国乌鸦（16，7.8％）。在这里，我们通过分子技术对加拿大不同蚊种的寄主摄食偏好进行了首次分析。我们关于蚊子分布和行为的研究结果将有助于开发有效的缓解和控制策略，以预防或减少有关病毒的人类/动物健康问题。