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Biological Applications of Synthesized ZnO Nanoparticles Using Pleurotus djamor Against Mosquito Larvicidal, Histopathology, Antibacterial, Antioxidant and Anticancer Effect
Journal of Cluster Science ( IF 2.8 ) Pub Date : 2020-11-05 , DOI: 10.1007/s10876-020-01927-z
Kumar Manimaran , Govindasamy Balasubramani , Chinnasamy Ragavendran , Devarajan Natarajan , Subban Murugesan

The present study pertained to biosynthesis, characterization and biomedical application (larvicidal, histopathology, antibacterial, antioxidant and anticancer activity) of Zinc oxide nanoparticles (ZnONPs) from Pleurotus djamor. The synthesized NPs were characterized using spectral and microscopic analyses and further confirmed by UV–Visible spectrophotometer with apeak of 350 nm. The ZnONPs showed strong antioxidant property (DPPH, H2O2 and ABTS+ radical assay) and expressed good larval toxicity against Ae. aegypti and Cx. quinquefasciatus (IVth instar larvae) with the least LC50 and LC90 values (10.1, 25.6 and 14.4, 31.7 mg/l) after 24 h treatment, respectively. We noticed the morphological changes (damaged anal papillae area and the cuticle layers) in the treated larvae. For the antibacterial assay, the highest growth inhibition zone was recorded in C. diphteriae (28.6 ± 0.3 mm), followed by P. fluorescens (27 ± 0.5 mm) and S. aureus (26.6 ± 1.5 mm). The in vitro cytotoxicity assay depicted a significant level of cytotoxic effects (LC50 values 42.26 μg/ml) of ZnONPs against the A549 lung cancer cells, even at low dose. The overall findings of the study suggest that P. djamor had the ability for the biosynthesis of ZnONPs and could act as an alternative biomedical agent for future therapeutic applications in medical avenues.



中文翻译:

杏鲍菇合成的ZnO纳米粒子在蚊子幼虫,组织病理学,抗菌,抗氧化和抗癌作用中的生物学应用

本研究涉及来自侧耳的氧化锌纳米颗粒(ZnONPs)的生物合成,表征和生物医学应用(杀幼虫,组织病理学,抗菌,抗氧化和抗癌活性)。使用光谱和显微镜分析对合成的NP进行表征,并通过可见光谱为350 nm的紫外可见分光光度计进一步确认。ZnONPs具有很强的抗氧化性能(DPPH,H 2 O 2和ABTS +自由基检测),并且对Ae具有良好的幼虫毒性aegyptiCx。LC 50和LC 90最少的quinquefasciatus(四龄幼虫)处理24小时后分别达到(10.1、25.6和14.4、31.7 mg / l)。我们注意到处理后的幼虫的形态变化(受损的肛门乳头区域和表皮层)。对于抗菌测定,最高的生长抑制区记录在双歧杆菌(28.6±0.3 mm),然后是荧光假单胞菌(27±0.5 mm)和金黄色葡萄球菌(26.6±1.5 mm)。体外细胞毒性试验表明,即使在低剂量下,ZnONPs对A549肺癌细胞也具有显着水平的细胞毒性作用(LC 50值为42.26μg/ ml)。该研究的总体发现表明,P。djamor 具有ZnONPs的生物合成能力,并可以作为替代生物医学试剂,用于将来在医疗领域的治疗应用。

更新日期:2020-11-05
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