当前位置: X-MOL 学术Braz. J. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immunological and bacteriological shifts associated with a flagellin-hyperproducing Salmonella Enteritidis mutant in chickens
Brazilian Journal of Microbiology ( IF 2.2 ) Pub Date : 2020-11-04 , DOI: 10.1007/s42770-020-00399-7
Fernanda de Oliveira Barbosa 1 , Oliveiro Caetano de Freitas Neto 2 , Lucas Bocchini Rodrigues Alves 1 , Valdinete Pereira Benevides 1 , Andrei Itajahy Secundo de Souza 1 , Marcela da Silva Rubio 1 , Adriana Maria de Almeida 1 , Mauro Mesquita Saraiva 1 , Celso José Bruno de Oliveira 3 , John Elmerdahl Olsen 4 , Angelo Berchieri Junior 1
Affiliation  

Salmonella Enteritidis causes infections in humans and animals which are often associated with extensive gut colonization and bacterial shedding in faeces. The natural presence of flagella in Salmonella enterica has been shown to be enough to induce pro-inflammatory responses in the gut, resulting in recruitment of polymorphonuclear cells, gut inflammation and, consequently, reducing the severity of systemic infection in chickens. On the other hand, the absence of flagellin in some Salmonella strains favours systemic infection as a result of the poor intestinal inflammatory responses elicited. The hypothesis that higher production of flagellin by certain Salmonella enterica strains could lead to an even more immunogenic and less pathogenic strain for chickens was here investigated. In the present study, a Salmonella Enteritidis mutant strain harbouring deletions in clpP and fliD genes (SE ΔclpPfliD), which lead to overexpression of flagellin, was generated, and its immunogenicity and pathogenicity were comparatively assessed to the wild type in chickens. Our results showed that SE ΔclpPfliD elicited more intense immune responses in the gut during early stages of infection than the wild type did, and that this correlated with earlier intestinal and systemic clearance of the bacterium.

中文翻译:

与鸡中鞭毛蛋白高产肠炎沙门氏菌突变体相关的免疫学和细菌学变化

肠炎沙门氏菌引起人类和动物感染,这通常与广泛的肠道定植和粪便中的细菌脱落有关。肠道沙门氏菌中鞭毛的天然存在已被证明足以在肠道中诱导促炎反应,导致多形核细胞的募集、肠道炎症,从而降低鸡全身感染的严重程度。另一方面,由于引发的肠道炎症反应不佳,一些沙门氏菌菌株中没有鞭毛蛋白有利于全身感染。这里研究了以下假设:某些沙门氏菌菌株产生的鞭毛蛋白更高,可能导致鸡的免疫原性更强、致病性更强的菌株。在目前的研究中,产生了一个含有 clpP 和 fliD 基因(SE ΔclpPfliD)缺失的肠炎沙门氏菌突变株,导致鞭毛蛋白的过度表达,并将其免疫原性和致病性与鸡的野生型进行了比较评估。我们的结果表明,在感染的早期阶段,SE ΔclpPfliD 在肠道中引发了比野生型更强烈的免疫反应,并且这与细菌的早期肠道和全身清除相关。
更新日期:2020-11-04
down
wechat
bug