Bacillus subtilis and Enterococcus faecium are commonly used probiotics. This study aimed to identify the effect of live combined Bacillus subtilis R0179 and Enterococcus faecium R0026 (LCBE) on obesity-associated hyperlipidaemia and gut microbiota in C57BL/6 mice. Forty male C57BL/6 mice were divided into four groups: normal group (N group), model group (M group), low dose group (L group), high dose group (H group). Mice gavaged with LCBE at 0.023 g/kg/mice/day (L group) or 0.23 g/kg/mice/day (H group) during fed with high-fat diet for 8 weeks. In vitroEnterococcus faecium R0026 had an ability to lower the low-concentration of cholesterol was 46%, ability to lower the high-concentration of cholesterol was 58%. LCBE significantly reduced the body weight gain, Lee index, brown fat index and body mass index of mice on high-fat diet. LCBE significantly improved serum lipids (including serum triglyceride, total cholesterol, low-density lipoprotein and high-density lipoprotein). LCBE significantly reduced liver total cholesterol. Serum lipopolysaccharide and total bile acid in L and H groups decreased significantly compared with M group. PCR-DGGE showed that the composition of gut microbiota in treatment groups was improved. Akkermansia muciniphila was found in H group. PCA result indicated a similar gut microbiota structure between LCBE treatment groups and normal group. Number of bands and Shannon diversity index increased significantly in LCBE treatment groups. Quantitative-PCR showed Bifidobacterium spp. increased significantly in H group compared with M group. LCBE alleviated liver steatosis and improved brown adipose tissue index.

中文翻译：

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粪肠球菌 R0026 与枯草芽孢杆菌 R0179 联合预防肥胖相关的高脂血症并调节 C57BL/6 小鼠的肠道菌群。

枯草芽孢杆菌和粪肠球菌是常用的益生菌。本研究旨在确定活联合枯草芽孢杆菌R0179 和屎肠球菌R0026 (LCBE) 对 C57BL/6 小鼠肥胖相关高脂血症和肠道菌群的影响。40只雄性C57BL/6小鼠分为4组：正常组（N组）、模型组（M组）、低剂量组（L组）、高剂量组（H组）。在高脂饮食喂养 8 周期间，小鼠以 0.023 g/kg/小鼠/天（L 组）或 0.23 g/kg/小鼠/天（H 组）灌胃 LCBE。体外粪肠球菌R0026降低低浓度胆固醇的能力为46%，降低高浓度胆固醇的能力为58%。LCBE显着降低高脂饮食小鼠的体重增加、Lee指数、棕色脂肪指数和体重指数。LCBE显着改善血脂（包括血清甘油三酯、总胆固醇、低密度脂蛋白和高密度脂蛋白）。LCBE 显着降低肝脏总胆固醇。L组和H组血清脂多糖和总胆汁酸较M组明显下降。PCR-DGGE显示治疗组的肠道菌群组成得到改善。阿克曼氏菌发现在H组。PCA 结果表明 LCBE 治疗组和正常组之间的肠道微生物群结构相似。LCBE 治疗组的条带数量和香农多样性指数显着增加。定量 PCR 显示双歧杆菌属。与 M 组相比，H 组显着增加。LCBE 减轻了肝脏脂肪变性并改善了棕色脂肪组织指数。