Hypoxia and stemness are important factors in tumor progression. We aimed to explore the ncRNA classifier associated with hypoxia and stemness in lung adenocarcinoma (LUAD). We found that the prognosis of LUAD patients with high hypoxia and stemness index was worse than that of patients with low hypoxia and stemness index. RNA expression profiles of these two clusters were analyzed, and 6867 differentially expressed (DE) mRNAs were screened. Functional analysis showed that DE mRNAs were associated with cell cycle and DNA replication. Protein–protein interaction network analysis revealed 20 hub genes, among which CENPF, BUB1, BUB1B, KIF23 and TTK had significant influence on prognosis. In addition, 807 DE lncRNAs and 243 DE miRNAs were identified. CeRNA network analysis indicated that AC079160.1-miR-539-5p-CENPF may be an important regulatory axis that potentially regulates the progression of LUAD. The expression of AC079160.1 and CENPF were positively correlated with hypoxia and stemness index, while miR-539-5p expression level was negatively correlated with hypoxia and stemness index. Overall, we identified CENPF, BUB1, BUB1B, KIF23 and TTK as potentially key genes involved in regulating hypoxia-induced tumor cell stemness, and found that AC079160.1-miR-539-5p-CENPF axis may be involved in regulating hypoxia induced tumor cell stemness in LUAD.

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缺氧和干度指数结合的lncRNA相关的ceRNA调控网络在肺腺癌中的构建和研究

缺氧和干性是肿瘤进展的重要因素。我们的目的是探讨与肺腺癌（LUAD）缺氧和干性相关的ncRNA分类器。我们发现低氧和干性指数高的LUAD患者的预后比低氧和干度指数低的患者的预后差。分析了这两个簇的RNA表达谱，并筛选了6867个差异表达（DE）mRNA。功能分析表明，DE mRNA与细胞周期和DNA复制有关。蛋白质-蛋白质相互作用网络分析揭示了20个中心基因，其中CENPF，BUB1，BUB1B，KIF23和TTK对预后有重要影响。另外，鉴定出807个DE lncRNA和243个DE miRNA。CeRNA网络分析表明为AC079160。1-miR-539-5p-CENPF可能是潜在的调控LUAD进展的重要调控轴。AC079160.1和CENPF的表达与缺氧和干度指数呈正相关，而miR-539-5p的表达水平与缺氧和干度指数呈负相关。总体而言，我们确定CENPF，BUB1，BUB1B，KIF23和TTK是参与调节缺氧诱导的肿瘤细胞干性的潜在关键基因，并发现AC079160.1-miR-539-5p-CENPF轴可能参与调节缺氧诱导的肿瘤LUAD中的细胞干。