Objective: Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes.
Methods: Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface.
Results: CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum.
Conclusion: PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.

Keywords: cubosomes, Ganoderma lucidum polysaccharide, surface-engineering, immune response, RNA-seq


中文翻译：

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表面工程立方体作为一种新型疫苗佐剂来调节先天免疫和提高体内适应性免疫

目的：最近的研究揭示了立方体的佐剂活性及其作为抗原递送系统的潜在用途。在这项研究中，为了进一步增强立方体的佐剂活性，在立方体表面修饰了两种阳离子聚合物。
方法：在这里，我们通过将两种分子，即十六烷基三甲基溴化铵 (CTAB) 和聚(二烯丙基二甲基氯化铵) (PDDAC)，利用表面化学对灵芝多糖立方体 (GLPC) 佐剂活性的影响。他们的表面。
结果：CTAB 或 PDDAC 修饰的 GLPC 被发现可显着促进体液和细胞免疫反应，以及通过强大的树突状细胞 (DC) 激活来促进 CD3+ CD4+ 或 CD3+ CD8+T 细胞的增殖。PDDAC 修饰的 GLPC 增强的免疫反应可能归因于 DCs 成熟为引流淋巴结和血清中脾脏和细胞因子的激活。
结论： PDDAC修饰有利于增强体液和细胞免疫反应，提示PDDAC-GLPC-OVA具有成为疫苗潜在佐剂的能力。

关键词： cubosomes，灵芝多糖，表面工程，免疫反应，RNA-seq