In this study, we compared new testate amoeba records produced by the conventional water-based wet-sieving method to testate amoeba data from previous palynological analyses of two Holocene peat profiles from British Columbia, Canada. We used 132 paired samples from the same peat cores to compare the composition of testate amoeba assemblages between the two methods and evaluate the potential of palynological testate amoeba records as quantitative paleohydrological proxies in peatlands. Our results suggest that the palynological treatments (acetolysis, 10% KOH and 150 µm sieving) substantially reduce test concentrations, diversity and richness in most pollen-slide samples. The smaller sieve size used in the palynological treatment (i.e. 150 µm) removed only a small fraction of the total tests, but could introduce a species-specific bias in assemblage composition. However, major shifts between wet and dry conditions, as shown by the water table depth (WTD) reconstructions using wet-sieved samples, were relatively well captured by the palynological datasets in most instances. The palynological assemblages with counts of >50 tests (n = 30) provided WTD estimates similar to those inferred from the wet-sieved samples as the most dominant hydrophilous and xerophilous taxa were relatively well represented in the pollen-slide samples in the two studied peat cores. Testate amoeba data from pollen slides can provide useful paleohydrological information particularly when combined with other paleoenvironmental proxies. Nonetheless, if the goal is to generate quantitative paleohydrological reconstructions, we recommend that testate amoeba analyses be conducted using the wet-sieving method, as testate amoeba concentrations, diversity and richness are typically much lower in samples prepared with palynological treatments.

中文翻译：

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全新世有遗嘱变形虫组合与花粉载玻片和湿筛泥炭古水文记录的比较

在这项研究中，我们比较了通过传统水基湿筛法产生的新的遗嘱变形虫记录与来自加拿大不列颠哥伦比亚省的两个全新世泥炭剖面的先前孢粉学分析的遗嘱变形虫数据。我们使用来自相同泥炭核心的 132 个配对样本来比较两种方法之间的遗嘱变形虫组合的组成，并评估孢粉学遗嘱变形虫记录作为泥炭地定量古水文代理的潜力。我们的结果表明孢粉处理（乙酰水解、10% KOH 和 150 µm 筛分）显着降低了大多数花粉载玻片样品的测试浓度、多样性和丰富度。孢粉处理中使用的较小筛网尺寸（即 150 µm）仅去除了总测试的一小部分，但可能会在组合组成中引入物种特定的偏差。然而，在大多数情况下，孢粉学数据集相对较好地捕获了湿条件和干条件之间的主要变化，如使用湿筛样本的地下水位深度 (WTD) 重建所示。计数 > 50 次测试 (n = 30) 的孢粉组合提供的 WTD 估计值与从湿筛样品推断的相似，因为最主要的亲水性和干旱性分类群在两个研究的泥炭的花粉载玻片样品中相对较好地代表核心。来自花粉幻灯片的遗嘱变形虫数据可以提供有用的古水文信息，尤其是在与其他古环境代理相结合时。尽管如此，如果目标是生成定量的古水文重建，