Nucleic acid aptamers are single-stranded oligonucleotides that may be evolved for affinity and specificity for their targets and can be easily produced, regenerated, and stabilized. In this study, we adapted Ni-NTA (nickle-charged nitrilotriacetic acid) affinity-chromatography in the development of single-stranded DNA aptamers against N-cadherin protein by systematic evolution of ligands by exponential enrichment (SELEX). After ten rounds of selection, two aptamers, designated NS13 and NC23, were selected, which showed low dissociation constants of 93 and 174 nM, respectively. The 5′-carboxyfluorescein-labeled NS13 was used for the sensitive detection of N-cadherin protein by the enzyme-linked oligonucleotide assay (ELONA) method.

中文翻译：

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用于选择 N-钙粘蛋白特异性 DNA 适体的 Ni-次氮基三乙酸亲和性 SELEX 方法

核酸适体是单链寡核苷酸，可以对其靶标具有亲和力和特异性，并且可以轻松生产、再生和稳定。在这项研究中，我们通过指数富集 (SELEX) 对配体的系统进化，在开发针对 N-钙粘蛋白的单链 DNA 适体的过程中调整了 Ni-NTA（带镍的次氮基三乙酸）亲和层析。经过十轮选择，选择了两个适体，命名为 NS13 和 NC23，分别显示出 93 和 174 nM 的低解离常数。5'-羧基荧光素标记的 NS13 用于通过酶联寡核苷酸测定 (ELONA) 方法灵敏检测 N-钙粘蛋白。