Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomer's mixture (1 : 1) (6α-hydroxy-7β-acetoxy-vouacapan-17β-oate methyl ester and 6α-acetoxy-7β-hydroxy-vouacapan-17β-oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF-α stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomer's mixture. P. pubescens extract (1.5625 - 25 µg/mL) and vouacapan diterpene furan isomer's mixture (3.125 - 50 µM) inhibited cell proliferation as indicated by a decreased BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125 - 12.5 µg/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 µM) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomer's mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomer's mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomer's mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach.

中文翻译：

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Pterodon pubescens 提取物和分离的二萜在 HaCaT 细胞中的抗增殖作用

Pterodon pubescens 果实因其镇痛和抗炎作用而被广泛使用，这归因于具有 vouacapan 骨架的分离化合物。这项工作旨在评估 P. pubescens 果实二氯甲烷提取物和 vouacapan 二萜呋喃异构体的混合物 (1:1)（6α-羟基-7β-乙酰氧基-vouacapan-17β-oate 甲酯和 6α -乙酰氧基-7β-羟基-vouacapan-17β-oate 甲酯异构体）在 HaCaT 细胞中使用细胞迁移和 BrDU 掺入测定。在TNF-α刺激后通过ELISA测量IL-8的水平。提取物的 HPLC/DAD 分析揭示了武阿卡潘二萜呋喃异构体混合物的明显存在。P. pubescens 提取物 (1.5625 - 25 µg/mL) 和 vouacapan 二萜呋喃异构体的混合物 (3. 125 - 50 µM) 抑制细胞增殖，如 BrdU 掺入减少所示。为了评估细胞迁移，使用了延时显微镜。P. pubescens 在所有测试浓度 (3.125 - 12.5 µg/mL) 下均表现出对细胞迁移的抑制作用，而对于 VDFI 混合物，仅在测试的最高浓度 (12.5 和 25 µM) 下观察到抑制作用。此外，P. pubescens 提取物和 vouacapan 二萜呋喃异构体的混合物显着降低了 IL-8 水平。我们的结果显示对用提取物和伏阿卡潘异构体混合物处理的 HaCaT 细胞具有抗增殖和抗炎作用，而不影响细胞活力。这些活性可归因于 voucapan 分子结构。总之，由 P. pubescens 提取物或 voucapan 异构体开发的外用产品