Membrane proteins are incredibly important biomolecules because they mediate interactions between a cell's external and internal environment. Obtaining information about membrane protein structure and interactions is thus important for understanding these essential biomolecules. Compared with the analyses of water-soluble proteins, the structural analysis of membrane proteins is more challenging owing to their unique chemical properties and the presence of lipid components that are necessary to solubilize them. The combination of covalent labeling (CL) and mass spectrometry (MS) has recently been applied with great success to study membrane protein structure and interactions. These studies have demonstrated the many advantages that CL-MS methods have over other traditional biophysical techniques. In this review, we discuss both amino acid-specific and non-specific labeling approaches and the special considerations needed to address the unique challenges associated with interrogating membrane proteins. This review highlights the aspects of this approach that require special care to be applied correctly and provides a comprehensive review of the membrane protein systems that have been studied by CL-MS. © 2020 John Wiley & Sons Ltd. Mass Spec Rev

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共价标记与质谱联用的膜蛋白结构和相互作用

膜蛋白是极其重要的生物分子，因为它们调节细胞内外环境之间的相互作用。因此，获得有关膜蛋白结构和相互作用的信息对于理解这些必需的生物分子非常重要。与水溶性蛋白质的分析相比，膜蛋白的结构分析由于其独特的化学性质和溶解它们所必需的脂质成分的存在而更具挑战性。共价标记 (CL) 和质谱 (MS) 的结合最近已成功应用于研究膜蛋白结构和相互作用。这些研究证明了 CL-MS 方法相对于其他传统生物物理技术的许多优势。在这篇评论中，我们讨论了氨基酸特异性和非特异性标记方法，以及解决与询问膜蛋白相关的独特挑战所需的特殊注意事项。这篇综述强调了这种方法需要特别小心才能正确应用的方面，并全面回顾了 CL-MS 研究的膜蛋白系统。© 2020 John Wiley & Sons Ltd. 质谱修订版