The yeast vacuole is functionally corresponding to vacuoles in eukaryote cells, it consists of a fusion protein that assists in the fusion of vacuoles and plays an important role in many processes. In addition, chemicals such as NH4Cl can reduce the size of vacuoles but as a side effect that also inhibits vacuoles making them inactive. In this study, to develop pre-treatments for extending the life of cut flowers, we constructed recombinant yeast using the fusion protein YPT7 and confirmed the activity of down-sized vacuoles. All the vacuoles of the recombinant yeast except vacuoles from recombinant yeast (MBTL-MYH-3) were found to be small vacuoles than mock (MBTL-MYH-0) and YPT7 overexpression model (MBTL-MYH-1). To confirm their activity, we conducted a test for antimicrobial activity. The results showed the other vacuoles of recombinant yeast had lower antimicrobial activity than the mock control, most of them showed about 60 % to 80 % of the antimicrobial activity. However, MBTL-MYH-3, whose vacuole did not change its size, showed antimicrobial activity lower than 40 %. Therefore, the cut flowers are better able to absorb smaller vacuoles after using the fusion protein YPT7. We expect that absorbing vacuoles more effective to senescence of cut flower than vacuolar enzymes.

中文翻译：

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YPT7 的缺失调节酵母液泡的活性

酵母液泡在功能上与真核细胞中的液泡相对应，它由一种融合蛋白组成，有助于液泡的融合，并在许多过程中发挥重要作用。此外，NH4Cl 等化学物质可以减小液泡的大小，但作为副作用也会抑制液泡，使其失去活性。在这项研究中，为了开发延长切花寿命的预处理，我们使用融合蛋白 YPT7 构建了重组酵母，并确认了缩小液泡的活性。除重组酵母（MBTL-MYH-3）的液泡外，重组酵母的所有液泡均比模拟（MBTL-MYH-0）和YPT7过表达模型（MBTL-MYH-1）的液泡小。为了确认它们的活性，我们进行了抗菌活性测试。结果表明，重组酵母的其他液泡的抗菌活性低于模拟对照，其中大部分表现出约 60% 至 80% 的抗菌活性。然而，MBTL-MYH-3 的液泡大小没有改变，其抗菌活性低于 40%。因此，切花在使用融合蛋白YPT7后能够更好地吸收较小的液泡。我们期望吸收液泡比液泡酶对切花衰老更有效。