Abstract In Martinique or La Reunion, French authorities recently banned the use of pesticides for the management of "soil-borne pathogens" on pineapple after several decades of intensive monoculture where the natural balance between beneficial and harmful communities of soil organisms has disappeared. Today, increasing infestation of pineapple by the nematode R. reniformis and other "soil-borne pathogens" causes severe damage to the crop. New cropping systems with innovative ecological nematode management are needed. An eco-friendly cropping system, which comprised rotation of sunn hemp (C. juncea), pineapple, a natural grass fallow and another cash crop, eggplant, consistently reduced the inoculum of nematodes. Nematode populations were reduced by 86.4%, 82% and 46.5% respectively under pineapple, sunn hemp and grass fallow compared to infestations of eggplant (3,456 nematodes per 100g of soil) after several rotations. Integrating a chemical induction of systemic resistance or application of an endophytic bacteria recovered from pineapple roots, Bacillus sp. GVS2, helped maintain low populations of nematodes during the vegetative cycle. On two pineapple varieties, MD2 and Victoria (Queen), two different treatments were applied monthly in the field, methyl jasmonate (10-4 M, 10 ml per plant), or a suspension of Bacillus sp. GVS2 (108 CFU.ml-1, 10 ml per plant) isolated from pineapple roots. After eight months, the nematode populations were reduced respectively on MD2 and Victoria (Queen) by 58.3% and 50.3% with the methyl jasmonate and by 59.6% and 54.3% with the Bacillus sp. GVS2 compared to controls. Because of the efficiency of sunn hemp in reducing the inoculum of nematodes, no significant differences in vegetative growth were observed using the D leaf weight and the estimated root length densities (RLD). The potential of pest management through eco-friendly cropping systems for pineapple with a biocontrol for nematodes with no pesticide is discussed.

中文翻译：

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结合轮作、化学介导抗性和内生细菌接种对菠萝上的肾形轮虫的田间管理

摘要 在马提尼克岛或留尼汪岛，法国当局最近禁止使用杀虫剂来管理菠萝上的“土传病原体”，因为经过几十年的集约化单一栽培，土壤生物有益和有害群落之间的自然平衡已经消失。今天，菠萝线虫 R. reniformis 和其他“土传病原体”对菠萝的侵染日益严重，对作物造成严重损害。需要具有创新生态线虫管理的新种植系统。一种生态友好的种植系统，包括轮作晒麻 (C. juncea)、菠萝、天然草休耕地和另一种经济作物茄子，不断减少线虫的接种量。菠萝下线虫数量分别减少了86.4%、82%和46.5%，与茄子的侵染（每 100 克土壤 3,456 条线虫）相比，经过几次轮换后，阳光下的大麻和草休耕。整合系统抗性的化学诱导或应用从菠萝根中回收的内生细菌，芽孢杆菌。GVS2 有助于在营养周期中维持低数量的线虫。在两个菠萝品种 MD2 和维多利亚（皇后）上，每月在田间应用两种不同的处理方法：茉莉酸甲酯（10-4 M，每株 10 毫升）或芽孢杆菌悬浮液。从菠萝根中分离的 GVS2（108 CFU.ml-1，每株植物 10 ml）。八个月后，MD2 和 Victoria (Queen) 上的线虫种群使用茉莉酸甲酯分别减少了 58.3% 和 50.3%，使用芽孢杆菌减少了 59.6% 和 54.3%。GVS2 与对照相比。由于 sunn 大麻在减少线虫接种方面的效率，使用 D 叶重和估计的根长密度 (RLD) 未观察到营养生长的显着差异。讨论了通过生态友好的菠萝种植系统进行病虫害管理的潜力，该系统对线虫进行生物防治而无需杀虫剂。