The aim of the work was to determine the effects of the chemical functionalization of hyaluronic acid (HA) with pendant aliphatic tails at different lengths and free amino groups in terms of chemical reactivity, degradation rate, drug-eluting features, and surface properties when processed as electrospun membranes (EM) evaluating the osteoinductive potential for a possible application as guided bone regeneration (GBR).

To this end, a series of HA derivatives with different aliphatic tails (DD-Cx mol% ≈ 12.0 mol%) and decreasing derivatization of free amino groups (DD_EDA mol% from 70.0 to 30.0 mol%) were first synthesized, namely Hn. Then dexamethasone-loaded Hn EM, i.e. HnX were prepared from aqueous polymeric solutions with polyvinyl alcohol (PVA), as a non-ionogenic linear flexible polymeric carrier, and the multifunctional 2-hydroxypropyl- cyclodextrin (HPCD) which acted as a rheological modifier, a stabilizer of Taylor’s cone, and a solubilizing agent. A comprehensive characterization of the membranes was carried out through ATR-IR, XRD, and WCA measurements. According to the in vitro hydrolytic and enzymatic degradation and drug release in different aqueous media for two months, the insertion of alkyl pendant grafts and the crosslinking process provided tuneable additional resistance to the whole membrane suitably for the final application of the membranes. Cell culture showed the cytocompatibility and cell proliferation until 7 days. Osteogenic differentiation and mineralization of pre-osteoblastic MC3T3 cells occurred for most of membranes after 35 days as valued by measuring ALP activity (50 nmol 4-np/h/nf DNA) and the deposition of calcium (120−140 μg ml⁻¹).

这项工作的目的是确定具有不同长度和游离氨基的脂肪族侧链的透明质酸（HA）的化学功能化对化学反应性，降解率，药物洗脱特征和加工后表面性质的影响作为静电纺丝膜（EM）的评估骨诱导潜力的潜在应用作为引导的骨再生（GBR）。

为此，首先合成了一系列具有不同脂族尾巴（DD-Cx mol％≈12.0 mol％）和游离氨基衍生化降低的HA衍生物（DD _EDA mol％从70.0降至30.0 mol％），即H n。然后加载地塞米松的H n EM，即H nX是由含聚乙烯醇（PVA）的聚合物水溶液制成的，该聚乙烯醇作为非离子型线性柔性聚合物载体，而多官能的2-羟丙基-环糊精（HPCD）用作流变改性剂，泰勒锥的稳定剂和增溶剂。通过ATR-IR，XRD和WCA测量对膜进行了全面表征。根据在不同水介质中体外水解和酶促降解以及药物释放两个月的结果，烷基侧基接枝物的插入和交联过程为整个膜提供了可调节的附加阻力，适合最终应用。细胞培养显示细胞相容性和细胞增殖直至7天。^-1）。