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Spirosoma aureum sp. nov., and Hymenobacter russus sp. nov., radiation-resistant bacteria in Cytophagales order isolated from soil
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-11-04 , DOI: 10.1007/s10482-020-01492-5
Soohyun Maeng 1 , Yuna Park 1 , Joo Hyun Han 1 , Sang Eun Lee 1 , Jing Zhang 2 , Min-Kyu Kim 2 , In-Tae Cha 3 , Ki-Eun Lee 3 , Byoung-Hee Lee 3 , Myung Kyum Kim 1
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A Gram-stain-negative, aerobic, nonmotile, yellow-colored strain BT328T and Gram-stain-negative, aerobic, non-motile, red-colored strain BT18T were isolated from the soil collected in Korea. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain BT328T formed a distinct lineage within the family Spirosomaceae (order Cytophagales, class Cytophagia) and was most closely related to a member of the genus Spirosoma, Spirosoma terrae 15J9-4T (95.9% 16S rRNA gene sequence similarity). Optimal growth occurred at 25 °C, pH 7.0 and in the absence of NaCl. The predominant cellular fatty acids were summed feature 3 (C16:1 ω6c/C16:1 ω7c) and C16:1 ω5c. The major respiratory quinone was MK-7. The major polar lipid was phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BT18T formed a distinct lineage within the family Hymenobacteraceae (order Cytophagales, class Cytophagia, phylum Bacteroidetes) and was most closely related to members of the genus Hymenobacter, Hymenobacter knuensis 16F7C-2T (97.0% 16S rRNA gene sequence similarity). Optimal growth occurred at 25 °C and pH 7.0 without NaCl. The major fatty acids were iso-C15:0 and anteiso-C15:0. The major menaquinone was MK-7. The major polar lipid was phosphatidylethanolamine. Biochemical, chemotaxonomic and phylogenetic analyses indicated that strains BT328T and BT18T represents a novel bacterial species within the genus Spirosoma and Hymenobacter, respectively. For which the name Spirosoma aureum and Hymenobacter russus is proposed. The type strain of S. aureum is BT328T (=KCTC 72365T = NBRC 114506T) and the type strain of H. russus is BT18T (=KCTC 62610T = NBRC 114380T).



中文翻译:

金黄色螺旋藻 十一月。十一月,从土壤中分离出食丝藻类的抗辐射细菌

从韩国收集的土壤中分离出革兰氏阴性,好氧,非运动型,黄色菌株BT328 T和革兰氏阴性,好氧,非运动型,红色BT18 T菌株。基于16S rRNA基因序列的系统发育分析表明,菌株BT328 Ť形成的家庭内的不同谱系Spirosomaceae(顺序Cytophagales,类Cytophagia),并最密切相关的属的成员SpirosomaSpirosoma terrae 15J9-4 Ť(95.9%的16S rRNA基因序列相似性)。在25°C,pH 7.0和不存在NaCl的情况下,生长最佳。主要细胞脂肪酸的总和,特征3(C 16:1 ω 6 ç / C 16:1 ω 7 Ç)和C 16:1 ω 5 Ç。主要的呼吸醌为MK-7。主要的极性脂质是磷脂酰乙醇胺。基于16S rRNA基因序列的系统发育分析表明,菌株BT18 Ť形成的家庭内的不同谱系Hymenobacteraceae(顺序Cytophagales,类Cytophagia,门拟杆菌),并且与处女膜的细菌门氏杆菌16F7C-2 T(97.0%的16S rRNA基因序列相似性)最为相关。在没有NaCl的情况下,在25°C和pH 7.0时最佳生长。主要脂肪酸是异-C 15:0和前异-C 15:0。主要的甲萘醌是MK-7。主要的极性脂质是磷脂酰乙醇胺。生化,化学分类和系统发育分析表明,菌株BT328 Ť和BT18 Ť表示属内的新的细菌物种SpirosomaHymenobacter分别。为此名称金黄色螺旋藻提出了处女膜杆菌。金黄色葡萄球菌的类型菌株为BT328 T(= KCTC 72365 T  = NBRC 114506 T),鲁氏酵母的类型菌株为BT18 T(= KCTC 62610 T  = NBRC 114380 T)。

更新日期:2020-11-04
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