Vanilla, a popular flavour extracted from Vanilla planifolia pods was tested for its antibiotic modulatory activity against extensively drug-resistant (XDR) Pseudomonas aeruginosa clinical isolates. Cured vanilla pod extract (VPE) was found non-bactericidal even at high doses (> 2000 µg/mL), however, it modulated the activity of several antibiotics at a sub-inhibitory concentration of 500 µg/mL. This modulation activity of VPE was observed for last line antibiotic options such as meropenem and tigecycline, as well as commonly used antibiotics like ciprofloxacin, levofloxacin and chloramphenicol. Further, it was observed that VPE inhibited the activity of efflux pumps in XDR P. aeruginosa clinical isolates. GC-MS spectral analysis revealed the dominance of vanillin, furfuran and some short-chain fatty acids in VPE. Therefore, further studies on the constituent ingredients in VPE are recommended to identify the active compounds and use them as antibiotic modulators and efflux pump inhibitors.

Graphical abstract

Vanilla extract inhibits dominant efflux pump in Pseudomonas aeruginosa and modulates the activity of last line and commonly used antibiotics

中文翻译：

---

香草调节抗药性铜绿假单胞菌中抗生素的活性并抑制外排泵

测试了香草（一种从扁平香草荚中提取的流行香料）对广泛耐药（XDR）的铜绿假单胞菌临床分离株的抗生素调节活性。发现即使在高剂量（> 2000 µg / mL）下，固化的香草豆荚提取物（VPE）也没有杀菌作用，但是，在500 µg / mL的亚抑制浓度下，它可以调节几种抗生素的活性。对于最后一线抗生素选择（如美罗培南和替加环素）以及常用抗生素（如环丙沙星，左氧氟沙星和氯霉素），观察到了VPE的这种调节活性。此外，观察到VPE抑制了XDR铜绿假单胞菌的外排泵的活性。临床分离株。GC-MS光谱分析表明，香兰素，呋喃丹和一些短链脂肪酸在VPE中占主导地位。因此，建议对VPE中的成分进行进一步研究，以鉴定活性化合物并将其用作抗生素调节剂和外排泵抑制剂。

图形概要

香草提取物抑制铜绿假单胞菌的主要外排泵并调节最后一系和常用抗生素的活性