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Dielectrophoretic characterization of dendritic cell deformability upon maturation.
Biotechniques ( IF 2.7 ) Pub Date : 2020-11-03 , DOI: 10.2144/btn-2020-0126
Anoop Menachery 1 , Jiranuwat Sapudom 2 , Abhishek Vembadi 3 , Aseel Alatoom 2 , Jeremy Teo 2, 4 , Mohammad A Qasaimeh 3, 4
Affiliation  

We have developed a rapid technique for characterizing the biomechanical properties of dendritic cells using dielectrophoretic forces. It is widely recognized that maturing of dendritic cells modulates their stiffness and migration capabilities, which results in T-cell activation triggering the adaptive immune response. Therefore it is important to develop techniques for mechanophenotyping of immature and mature dendritic cells. The technique reported here utilizes nonuniform electric fields to exert a substantial force on the cells to induce cellular elongation for optical measurements. In addition, a large array of interdigitated electrodes allows multiple cells to be stretched simultaneously. Our results indicate a direct correlation between F-actin activity and deformability observed in dendritic cells, determined through mean fluorescence signal intensity of phalloidin.

中文翻译:

成熟时树突细胞变形能力的介电泳表征。

我们开发了一种使用介电泳力表征树突细胞生物力学特性的快速技术。人们普遍认为,树突状细胞的成熟会调节它们的硬度和迁移能力,这会导致 T 细胞激活触发适应性免疫反应。因此,重要的是开发未成熟和成熟树突细胞的机械表型分析技术。这里报道的技术利用非均匀电场对细胞施加很大的力,以诱导细胞伸长以进行光学测量。此外,大量交叉电极允许同时拉伸多个细胞。我们的结果表明在树突细胞中观察到的 F-肌动蛋白活性和变形能力之间存在直接相关性,
更新日期:2020-11-05
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