Nanostructures synthesized using DNA-conjugated gold nanoparticles have a wide range of applications in the field of biosensorics. The stability of the DNA duplex plays a critical role as it determines the final geometry of these nanostructures. The main way to control DNA stability is to maintain a high ionic strength of the buffer solution; at the same time, high salt concentrations lead to an aggregation of nanoparticles. In this study, by means of the instrumentality of DNA-bridged seeds using tris(hydroxymethyl)aminomethane as a soft reducing agent the dumbbell-like gold nanoparticles up to 35 nm were synthesized with a high concentration of sodium ions of up to 100 mM and magnesium ions up to 1 mM. We also examined at the atomic level the details of the effect of the gold nanoparticle surface, as well as Na+ and Mg2+ ions, on the stability of nucleotide pairs located in close proximity to the grafting site.

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DNA 桥接金纳米粒子合成过程中钠和镁离子对 DNA 的稳定作用

使用 DNA 共轭金纳米粒子合成的纳米结构在生物传感领域具有广泛的应用。DNA 双链体的稳定性起着至关重要的作用，因为它决定了这些纳米结构的最终几何形状。控制DNA稳定性的主要方法是保持缓冲液的高离子强度；同时，高盐浓度会导致纳米颗粒聚集。在这项研究中，通过使用三（羟甲基）氨基甲烷作为软还原剂的 DNA 桥接种子的工具，合成了高达 35 nm 的哑铃状金纳米颗粒，钠离子浓度高达 100 mM，并且镁离子高达 1 mM。我们还在原子水平上检查了金纳米颗粒表面以及 Na+ 和 Mg2+ 离子的影响细节，