Ever-increasing attention has been focused on constructing a sensing system for specific detection of glutathione (GSH) over cysteine (Cys) and homocysteine (Hcy), which usually interfere with the GSH detection due to their similar structures and the presence of thiol groups in these amino acids. Here, a novel fluorescence-sensing system is developed for highly specific GSH detection over Cys and Hcy. The sensing system is constructed through facilely mixing dipicolinic acid (DPA) and guanosine 5′-monophosphate (GMP) with cerium acetate at ambient conditions, denoted as DPA-Ce-GMP. The resultant DPA-Ce-GMP possesses fluorescence emission with excellent thermal stability and anti-light bleaching, which can be quenched by copper ions (Cu²⁺). The GSH, and not Cys or Hcy, can trap Cu²⁺ from DPA-Ce-GMP, resulting in the restoration of the fluorescence of the sensing system. The limit of detection reaches as low as 7.1 nM. The GSH detection in a real sample of human serum was further explored and exhibits satisfactory recovery. The developed sensing system has the advantages of ease-of-preparation, excellent selectivity and stability, demonstrating its potential application in disease diagnosis in the future.

中文翻译：

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一种基于铈的荧光纳米传感器，用于高特异性检测半胱氨酸和同型半胱氨酸上的谷胱甘肽

越来越多的注意力集中在构建用于特异性检测谷胱甘肽（GSH）而不是半胱氨酸（Cys）和高半胱氨酸（Hcy）的传感系统，由于它们的相似结构和硫醇基团的存在，这通常会干扰GSH的检测。这些氨基酸。在这里，开发了一种新型荧光传感系统，用于对 Cys 和 Hcy 进行高度特异性的 GSH 检测。该传感系统是通过在环境条件下将吡啶二羧酸 (DPA) 和鸟苷 5'-一磷酸 (GMP) 与乙酸铈轻松混合构建的，表示为 DPA-Ce-GMP。所得DPA-Ce-GMP具有荧光发射，具有优异的热稳定性和抗光漂白性，可被铜离子（Cu ²⁺）淬灭。GSH，而不是 Cys 或 Hcy，可以捕获 Cu ²⁺来自 DPA-Ce-GMP，导致传感系统的荧光恢复。检测限低至 7.1 nM。进一步探索了真实人血清样品中的 GSH 检测并显示出令人满意的回收率。所开发的传感系统具有制备简便、选择性好、稳定性好等优点，未来有望应用于疾病诊断。