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Standardization of Microcomputed Tomography for Tracheal Tissue Engineering Analysis
Tissue Engineering, Part C: Methods ( IF 3 ) Pub Date : 2020-11-17 , DOI: 10.1089/ten.tec.2020.0211
Jakob M Townsend 1 , Robert A Weatherly 2 , Jed K Johnson 3 , Michael S Detamore 4
Affiliation  

Tracheal tissue engineering has become an active area of interest among clinical and scientific communities; however, methods to evaluate success of in vivo tissue-engineered solutions remain primarily qualitative. These evaluation methods have generally relied on the use of photographs to qualitatively demonstrate tracheal patency, endoscopy to image healing over time, and histology to determine the quality of the regenerated extracellular matrix. Although those generally qualitative methods are valuable, they alone may be insufficient. Therefore, to quantitatively assess tracheal regeneration, we recommend the inclusion of microcomputed tomography (μCT) to quantify tracheal patency as a standard outcome analysis. To establish a standard of practice for quantitative μCT assessment for tracheal tissue engineering, we recommend selecting a constant length to quantify airway volume. Dividing airway volumes by a constant length provides an average cross-sectional area for comparing groups. We caution against selecting a length that is unjustifiably large, which may result in artificially inflating the average cross-sectional area and thereby diminishing the ability to detect actual differences between a test group and a healthy control. Therefore, we recommend selecting a length for μCT assessment that corresponds to the length of the defect region. We further recommend quantifying the minimum cross-sectional area, which does not depend on the length, but has functional implications for breathing. We present empirical data to elucidate the rationale for these recommendations. These empirical data may at first glance appear as expected and unsurprising. However, these standard methods for performing μCT and presentation of results do not yet exist in the literature, and are necessary to improve reporting within the field. Quantitative analyses will better enable comparisons between future publications within the tracheal tissue engineering community and empower a more rigorous assessment of results.

中文翻译:

用于气管组织工程分析的微计算机断层扫描的标准化

气管组织工程已成为临床和科学界的一个活跃领域。然而,评估体内成功的方法组织工程解决方案仍然主要是定性的。这些评估方法通常依赖于使用照片来定性证明气管通畅、内窥镜检查随着时间的推移图像愈合,以及组织学来确定再生细胞外基质的质量。尽管那些通常的定性方法很有价值,但仅靠它们可能是不够的。因此,为了定量评估气管再生,我们建议将微型计算机断层扫描 (μCT) 纳入量化气管通畅度作为标准结果分析。为了建立气管组织工程定量 μCT 评估的实践标准,我们建议选择恒定长度来量化气道容积。将气道容积除以恒定长度提供平均用于比较组的横截面积。我们警告不要选择过大的长度,这可能会导致人为地夸大平均横截面积,从而降低检测测试组和健康对照之间实际差异的能力。因此,我们建议选择与缺陷区域长度相对应的 μCT 评估长度。我们进一步建议量化最低横截面积,不取决于长度,但对呼吸有功能影响。我们提供经验数据来阐明这些建议的基本原理。这些经验数据乍一看似乎符合预期且不足为奇。然而,这些用于执行 μCT 和结果呈现的标准方法在文献中尚不存在,并且对于改进该领域内的报告是必要的。定量分析将更好地促进气管组织工程界未来出版物之间的比较,并能够对结果进行更严格的评估。
更新日期:2020-11-18
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