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Evaluation of Interleukin-23 and JAKs/STATs/SOCSs/ROR-γt Expression in Type 2 Diabetes Mellitus Patients Treated With or Without Sitagliptin
Journal of Interferon & Cytokine Research ( IF 2.3 ) Pub Date : 2020-11-16 , DOI: 10.1089/jir.2020.0113
Mahsa Rezaeepoor 1 , Mirhamed Hoseini-Aghdam 1 , Vida Sheikh 2 , Mohammad Mahdi Eftekharian 1 , Mahdi Behzad 1
Affiliation  

The production of interleukin-23 (IL-23) and the expression levels of related genes were evaluated in type 2 diabetes mellitus patients. The correlations between them were also determined. Thirty patients without sitagliptin (sitagliptin negative; SN), 30 patients with sitagliptin (sitagliptin positive; SP), and 30 healthy controls (HCs) were recruited. The level of IL-23 in the supernatant of anti CD3-activated peripheral blood mononuclear cells (PBMCs) was assessed using enzyme-linked immunosorbent assay. The expressions of IL-23, JAK1/JAK2/TYK2, STAT1/STAT3, ROR-γt, and SOCS1/SOCS3 in PBMCs were evaluated by real-time polymerase chain reaction. The production of IL-23 and the expressions of IL-23, JAK2, STAT3, and ROR-γt were observed to be enhanced in SN patients versus HCs, while the levels were decreased in SP patients versus SN patients (P < 0.05). SOCS1 and SOCS3 expressions were lower in SN patients than HCs, and their expressions were elevated in SP patients versus SN patients (P < 0.05). In SN patients, positive correlations between the IL-23 with fasting plasma glucose and HbA1c were observed, and JAK2/STAT3/ROR-γt were positively correlated with IL-23. JAK2, STAT3, and ROR-γt were positively related to each other and were negatively related to SOCS3. Enhanced IL-23/JAK2/STAT3/ROR-γt and reduced SOCS1/SOCS3 were found in SN patients. Sitagliptin may regulate the IL-23 and related gene expression.

中文翻译:

用或不用西格列汀治疗的 2 型糖尿病患者中白细胞介素 23 和 JAKs/STATs/SOCSs/ROR-γt 表达的评价

在 2 型糖尿病患者中评估了白细胞介素 23 (IL-23) 的产生和相关基因的表达水平。还确定了它们之间的相关性。招募了 30 名没有西他列汀的患者(西他列汀阴性;SN)、30 名有西他列汀的患者(西他列汀阳性;SP)和 30 名健康对照(HC)。使用酶联免疫吸附测定法评估抗 CD3 激活的外周血单核细胞 (PBMC) 上清液中 IL-23 的水平。的表达IL-23JAK1 / JAK2 / TYK2STAT1 / STAT3ROR-γTSOCS1 / SOCS3在PBMC中通过实时聚合酶链反应进行评价。IL-23的产生及表达IL-23JAK2STAT3ROR-γt在 SN 患者中与HC相比升高,而在 SP 患者与 SN 患者中水平降低(P  < 0.05)。SOCS1SOCS3在 SN 患者中的表达低于 HC,在 SP 患者中的表达高于 SN 患者(P  < 0.05)。在SN患者中,观察到IL-23与空腹血糖和HbA1c呈正相关,JAK2/STAT3/ROR-γt与IL-23呈正相关。JAK2STAT3ROR-γt之间呈正相关,与SOCS3呈负相关。在 SN 患者中发现了增强的IL-23/JAK2/STAT3/ROR-γt和降低的SOCS1/SOCS3。西格列汀可能调节 IL-23 和相关基因的表达。
更新日期:2020-11-18
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