The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 10³ individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males.

通过性别分类和仅释放不育雄性，提高了不育昆虫技术（SIT）的田间性能。这可以通过形态上的资源密集型分离男性和女性来完成。或者，可以使用性别比偏向的遗传构建体来选择性去除一个性别，而无需手动或自动分类，但是所得的基因工程（GE）防治剂将受到其他政府管制。在这里，我们描述并证明了非GE男性批量生产的遗传方法。该方法可用于在任何具有染色体性别决定性的生物体中产生异配子性行为（XY或WZ）。我们观察到超过10 ^3的分批培养物高达100％的性别选择个人。使用严格的转基因检测方法，我们证明了无转基因雄性的大规模生产的潜力。