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Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements
ACS Sensors ( IF 8.9 ) Pub Date : 2020-11-03 , DOI: 10.1021/acssensors.0c01623
Reinier Oropesa-Nuñez 1 , Teresa Zardán Gómez de la Torre 1 , Henry Stopfel 1 , Peter Svedlindh 1 , Mattias Strömberg 1 , Klas Gunnarsson 1
Affiliation  

Understanding the binding mechanism between probe-functionalized magnetic nanoparticles (MNPs) and DNA targets or amplification products thereof is essential in the optimization of magnetic biosensors for the detection of DNA. Herein, the molecular interaction forming hybrid structures upon hybridization between DNA-functionalized magnetic nanoparticles, exhibiting Brownian relaxation, and rolling circle amplification products (DNA-coils) is investigated by the use of atomic force microscopy in a liquid environment and magnetic biosensors measuring the frequency-dependent magnetic response and the frequency-dependent modulation of light transmission. This approach reveals the qualitative and quantitative correlations between the morphological features of the hybrid structures with their magnetic response. The suppression of the high-frequency peak in the magnetic response and the appearance of a new peak at lower frequencies match the formation of larger sized assemblies upon increasing the concentration of DNA-coils. Furthermore, an increase of the DNA-coil concentration induces an increase in the number of MNPs per hybrid structure. This study provides new insights into the DNA–MNP binding mechanism, and its versatility is of considerable importance for the mechanistic characterization of other DNA-nanoparticle biosensor systems.

中文翻译:

DNA-磁性纳米粒子杂化结构形成的见解:形态特征与磁性生物传感器测量结果之间的相关性

了解探针功能化的磁性纳米粒子(MNP)与DNA靶标或其扩增产物之间的结合机制,对于优化用于检测DNA的磁性生物传感器至关重要。本文中,通过在液体环境中使用原子力显微镜和磁性生物传感器测量频率,研究了在DNA功能化的磁性纳米粒子之间表现出布朗弛豫和滚动圆环扩增产物(DNA线圈)之间杂交形成杂交结构的分子相互作用。磁响应和光传输的频率调制。这种方法揭示了混合结构的形态特征与其磁响应之间的定性和定量相关性。磁响应中的高频峰的抑制和较低频率处的新峰的出现与DNA线圈浓度的增加形成了更大尺寸的组件。此外,DNA-线圈浓度的增加导致每个杂种结构的MNP数量增加。这项研究提供了对DNA-MNP结合机制的新见解,其多功能性对于其他DNA纳米颗粒生物传感器系统的机械表征具有相当重要的意义。
更新日期:2020-11-25
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