Abstract

To mimic the fibrous architecture of collagen, the nanofibrous gelatin scaffolds are fabricated employing a thermally induced phase separation (TIPS) technique. The influences of processing parameters, including polymer concentration and solvent mixture composition on the scaffold microstructure are investigated. However, using the TIPS technique, a limited pore size range is generally obtained. To yield the well-interconnected macroporous structures with equiaxed pores and nanofibrous architectures, the TIPS technique is combined with particulate leaching. The macroporous structure of produced scaffolds duplicates the predefined three-dimensional template structure. The homogenous macrostructure with well-interconnected equiaxed pores and no particular orientation is created. Modulating the size and shape of microspheres has precise control over porosity, pore size, and interconnection of the matrix. Because of the well-interconnected macroporous nanofibrous structure, the useful applications of these scaffolds in the tissue engineering field are expected.

摘要

为了模拟胶原蛋白的纤维结构，纳米纤维明胶支架采用热致相分离 (TIPS) 技术制造。研究了加工参数，包括聚合物浓度和溶剂混合物组成对支架微观结构的影响。然而，使用 TIPS 技术，通常会获得有限的孔径范围。为了产生具有等轴孔和纳米纤维结构的良好互连的大孔结构，TIPS 技术与颗粒浸出相结合。生产的支架的大孔结构复制了预定义的三维模板结构。具有良好互连的等轴孔且未产生特定取向的均质宏观结构。调节微球的大小和形状可以精确控制基质的孔隙率、孔径和互连。由于具有良好互连的大孔纳米纤维结构，这些支架在组织工程领域的有用应用值得期待。