Abstract

Due to the high demand, orchid propagation by in vitro techniques has lately increased, with an emphasis on the ways to improve germination and seedling development. In this work we compare the efficiency of three classic media for orchid propagation with a newly formulated medium supplemented with almond milk. Our target species responded differently to both the media and the supplement. However, with the exception of Bulbophyllum plumatum that did not show statistical differences with the most common media, a beneficial effect on orchid development growing on the new supplemented medium was observed for Cymbidium tracyanum, Dendrobium wardianum and for the two European orchid species Dactylorhiza praetermissa and Himantoglossum robertianum, speeding up the development of the latter two species into photosynthetic stages. The new medium could be, therefore, suitable for improving the development of some ornamental orchids and terrestrial orchid seedlings included in conservation projects that need an ex situ approach.

摘要

由于需求量大，最近通过体外技术繁殖兰花有所增加，重点是改善发芽和幼苗发育的方法。在这项工作中，我们比较了三种经典培养基的兰花繁殖效率和一种新配制的添加杏仁奶的培养基。我们的目标物种对媒体和补充的反应不同。然而，除了与最常见的培养基没有显示出统计学差异的Bulbophyllum plumatum之外，观察到Cymbidium tracyanum、Dendrobiumwardianum和两种欧洲兰花Dactylorhiza praetermissa和Himantoglossum robertianum，加速后两个物种进入光合作用阶段。因此，新培养基可能适用于改善一些需要迁地保护项目中的观赏兰花和陆生兰花幼苗的发育。