Expert Review of Proteomics ( IF 3.4 ) Pub Date : 2020-11-12 , DOI: 10.1080/14789450.2020.1846524 Masahiro Sugimoto 1, 2
ABSTRACT
Introduction: Saliva is an ideal biofluid that can be collected in a noninvasive manner, enabling safe and frequent screening of various diseases. Recent studies have revealed that salivary metabolomics analysis has the potential to detect both oral and systemic cancers.
Area covered: We reviewed the technical aspects, as well as applications, of salivary metabolomics for cancer detection. The topics include the effects of preconditioning and the method of sample collection, sample storage, processing, measurement, data analysis, and validation of the results. We also examined the rational relationship between salivary biomarkers and tumors distant from the oral cavity. A strategy to establish standard operating protocols for obtaining reproducible quantification data is also discussed
Expert opinion: Salivary metabolomics reflects oral and systematic health status, which potently enables cancer detection. The sensitivity and specificity of each marker and their combinations have been well evaluated, but a validation study is required. Further, the standard operating protocol for each procedure should be established to obtain reproducible data before clinical usage.
中文翻译:
用于癌症检测的唾液代谢组学
摘要
简介: 唾液是一种理想的生物体液,可以以无创方式收集,可以安全、频繁地筛查各种疾病。最近的研究表明,唾液代谢组学分析具有检测口腔和全身癌症的潜力。
涵盖的领域:我们回顾了唾液代谢组学在癌症检测中的技术方面和应用。主题包括预处理的影响和样本收集方法、样本存储、处理、测量、数据分析和结果验证。我们还研究了唾液生物标志物与远离口腔的肿瘤之间的合理关系。还讨论了建立标准操作协议以获得可重复的定量数据的策略
专家意见:唾液代谢组学反映口腔和系统健康状况,可有效地检测癌症。每个标志物及其组合的敏感性和特异性已得到很好的评估,但需要进行验证研究。此外,应建立每个程序的标准操作协议,以便在临床使用前获得可重复的数据。