Autophagy is a highly conserved protein degradation pathway that is essential for affecting some autoimmune diseases. Immune thrombocytopenia (ITP) is a common autoimmune disorder, and the complex dysregulation of cellular immunity has been observed; however, the relationship between autophagy‐related proteins and immune responses in ITP remains unclear. Using real‐time quantitative polymerase chain reaction (RT‐PCR), the mRNA expression levels of Beclin‐1, SQSTM1/p62 and LC3 were measured in the peripheral blood mononuclear cells (PBMCs) of 20 newly diagnosed patients with active ITP, 16 ITP patients in remission and 21 healthy volunteers. The stained Beclin‐1 and SQSTM1/p62 proteins were also observed in the bone marrow of active ITP patients and normal controls by immunofluorescence. SQSTM1/p62 mRNA expression in PBMCs in newly diagnosed patients was significantly decreased. At the same time, Beclin‐1 mRNA was increased significantly. During the remission stages, the levels of these autophagy‐related proteins were comparable with those observed in healthy controls. Taken together, these results suggest that the aberrant expression of autophagy‐related proteins might be involved in the pathogenesis of ITP. Further study of the autophagy pathway may provide a new strategy and direction for the treatment of ITP.

中文翻译：

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自噬相关蛋白在免疫性血小板减少症中的异常表达

自噬是高度保守的蛋白质降解途径，对于影响某些自身免疫性疾病至关重要。免疫性血小板减少症（ITP）是一种常见的自身免疫性疾病，并且已经观察到细胞免疫的复杂失调。然而，ITP中自噬相关蛋白与免疫反应之间的关系仍不清楚。使用实时定量聚合酶链反应（RT-PCR），在20例新确诊的ITP活跃患者，16例ITP患者的外周血单个核细胞（PBMC）中测量Beclin-1，SQSTM1 / p62和LC3的mRNA表达水平缓解患者和21名健康志愿者。通过免疫荧光在活跃的ITP患者和正常对照者的骨髓中也观察到染色的Beclin-1和SQSTM1 / p62蛋白。新诊断患者的PBMC中SQSTM1 / p62 mRNA表达明显降低。同时，Beclin-1 mRNA显着增加。在缓解阶段，这些自噬相关蛋白的水平与健康对照组中观察到的水平相当。综上所述，这些结果表明自噬相关蛋白的异常表达可能与ITP的发病机制有关。自噬途径的进一步研究可能为ITP的治疗提供新的策略和方向。这些结果表明自噬相关蛋白的异常表达可能与ITP的发病机制有关。自噬途径的进一步研究可能为ITP的治疗提供新的策略和方向。这些结果表明自噬相关蛋白的异常表达可能与ITP的发病机制有关。自噬途径的进一步研究可能为ITP的治疗提供新的策略和方向。