Abstract In this study, glassy carbon electrode(GCE) modified with zinc oxide nanoparticles (ZnO NPs) sensor was developed for paracetamol(PRM) detection. This antipyretic, anti-inflammatory drug usage is ample in medical field and over dose, continuing use for a long time affects kidney and liver because of accumulation of metabolite, hence paracetamol detection is significant. Cyclic voltametry (CV) and differential pulse voltametric (DPV) techniques were employed to analyse the behaviour of analyte on modified GCE in 0.2 M phosphate buffer solution (PBS) of physiological pH. ZnONP/GCE showed good linear response in the concentration range from 40.0 × 10−8 M to 24.0 × 10−6 M for quantitative determination of PRM with lower limit of detection of 3.6 nM. Developed technique was successfully applied to assay the content labelled in different PRM tablets. It also exhibited good selectivity, after series of common physiological intereferent's assessments. As a proof of concept, modified electrode was investigated in PRM spiked human urine samples.

中文翻译：

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用于灵敏测定扑热息痛的氧化锌纳米颗粒修饰玻碳电极的制备和表征

摘要 本研究开发了氧化锌纳米颗粒（ZnO NPs）传感器修饰的玻碳电极（GCE），用于检测扑热息痛（PRM）。这种解热消炎药在医学领域使用量大，剂量过大，长期持续使用会因代谢产物蓄积而影响肾脏和肝脏，因此对乙酰氨基酚检测意义重大。采用循环伏安法 (CV) 和微分脉冲伏安法 (DPV) 技术来分析分析物在生理 pH 值的 0.2 M 磷酸盐缓冲溶液 (PBS) 中的改良 GCE 上的行为。ZnONP/GCE 在 40.0 × 10-8 M 到 24.0 × 10-6 M 的浓度范围内显示出良好的线性响应，用于定量测定 PRM，检测下限为 3.6 nM。开发的技术已成功应用于检测不同 PRM 片剂中标记的含量。经过一系列常见的生理干扰评估后，它还表现出良好的选择性。作为概念证明，我们在掺有 PRM 的人类尿液样本中研究了改良电极。