The effects of two plant volatile compounds (VOCs), safranal and tulipalin A were investigated using the protoplast method with digital image analysis, which was developed to investigate allelopathic activities of plants at the cellular level in a 50 μL liquid medium. Both VOCs showed inhibition on lettuce protoplasts growth at all three stages, i.e., the cell wall formation stage, the cell division stage, and the yellow colour accumulation stage. Among the three stages of protoplast growth, differences of inhibition patterns were observed. Inhibitions at the cell wall formation stage were stronger than those at the latter two stages in both VOCs. Tulipalin A showed higher inhibitory activity (total inhibition at 100 μM) than that of safranal (44% inhibition at 1 mM) at cell division stage. They were also compared with the direct exposure method in an enclosed vial using germinated lettuce seedlings, which was developed to investigate allelopathic activities of VOCs at plant level. Tulipalin A showed less inhibitory activity on radicle growth of lettuce, than that of safranal reported. Inhibition on hypocotyl growth was stronger than that on radicle in both VOCs. The causes of the differences between two VOCs and two bioassay methods, and the application of the protoplast method, were discussed for clarifying the contribution of VOCs as allelochemicals at cellular level.

使用原生质体方法和数字图像分析研究了两种植物挥发性化合物（VOC）的safranal和tulipalin A的作用，该方法用于研究50μL液体培养基中细胞水平上植物的化感活性。在所有三个阶段，即细胞壁形成阶段，细胞分裂阶段和黄色累积阶段，两种VOC均显示出对莴苣原生质体生长的抑制作用。在原生质体生长的三个阶段中，观察到抑制模式的差异。在两种挥发性有机化合物中，细胞壁形成阶段的抑制作用均强于后两个阶段。在细胞分裂阶段，郁金香醛A的抑制活性（在100μM时的总抑制率）高于在胚泡中（在1 mM时的抑制率44％）。还使用发芽的生菜幼苗在封闭的小瓶中将它们与直接暴露方法进行了比较，该方法用于研究植物水平上VOC的化感活性。郁金香醛A对生菜的胚根生长的抑制活性要比sa虫的抑制作用小。在两种挥发性有机化合物中，对下胚轴生长的抑制作用均强于对胚根的抑制作用。讨论了两种挥发性有机化合物和两种生物测定方法之间差异的原因以及原生质体方法的应用，以阐明挥发性有机化合物在细胞水平上作为化感物质的贡献。在两种挥发性有机化合物中，对下胚轴生长的抑制作用均强于对胚根的抑制作用。讨论了两种挥发性有机化合物和两种生物测定方法之间差异的原因以及原生质体方法的应用，以阐明挥发性有机化合物在细胞水平上作为化感物质的贡献。在两种挥发性有机化合物中，对下胚轴生长的抑制作用均强于对胚根的抑制作用。讨论了两种挥发性有机化合物和两种生物测定方法之间差异的原因以及原生质体方法的应用，以阐明挥发性有机化合物在细胞水平上作为化感物质的贡献。