In this study, the chemical profile, antioxidative, cytotoxic and apoptotic effects of essential oil of Paracaryum bingoelianum Behçet &İlçim (Boraginaceae) were investigated in vitro. The chemical composition of P. bingoelianum essential oil (EO) was determined by HS-SPME/GC–MS method. DPPH free radical scavenging activity, ferric thiocyanate (FTC), and ferric reducing antioxidant potential (FRAP) methods were performed for revealing the antioxidant capacity of EO. The cytotoxic effect of EO was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) cell viability assay on MCF-7 breast cancer and HT-29 colorectal adenocarcinoma cells. Apoptosis inducing effect of EO was evaluated by cell death detection Elisa (CDDE) assay and acridine orange/ethidium bromide staining method. Totally thirty-four compounds, representing 93.38% of the essential oil were identified. The DPPH scavenging activity of EO at 205 μg/mL concentration, was found as close to vit. C. Besides, it was observed that EO had significant reducing power and total antioxidant capacity. The EO inhibited the proliferation of MCF-7 and HT-29 cells, and induced apoptosis. When compared with untreated cells, the viability of MCF-7 and HT-29 cells, which treated with 800 μg/mL EO, decreased to 48.9% and 36.2%, respectively. In treated MCF-7 and HT-29 cells with EO at IC₅₀ doses, the apoptosis was significantly induced, compared to untreated cells (p<0.0001). All these results suggest that P. bingoelianum contains important bioactive compounds, and might have antioxidant and antitumor activity.

在这项研究中，化学成分，抗氧化，细胞毒性和凋亡的影响，对油状的Paracaryum bingoelianumBehçet＆İlçim（Boraginaceae）进行了体外研究。P的化学组成。宾龙香精油（EO）通过HS-SPME / GC-MS方法测定。进行了DPPH自由基清除活性，硫氰酸铁（FTC）和铁还原抗氧化电位（FRAP）方法，以揭示EO的抗氧化能力。通过MTT（3- [4,5-二甲基噻唑-2-基] -2,5二苯基溴化四唑鎓）细胞活力测定法测定MC对MCF-7乳腺癌和HT-29结肠直肠腺癌细胞的EO细胞毒性作用。通过细胞死亡检测Elisa（CDDE）分析和a啶橙/溴化乙锭染色法评估EO的凋亡诱导作用。总共鉴定出34种化合物，占精油的93.38％。发现205μg/ mL浓度的EO的DPPH清除活性接近vit。C.此外，观察到，EO具有显着的还原能力和总抗氧化能力。EO抑制MCF-7和HT-29细胞的增殖，并诱导细胞凋亡。与未经处理的细胞相比，用800μg/ mL EO处理的MCF-7和HT-29细胞的活力分别下降至48.9％和36.2％。在经过处理的MCF-7和HT-29细胞中，EO在IC处与未处理的细胞相比，在₅₀剂量下，细胞凋亡得到了显着诱导（p <0.0001）。所有这些结果表明，P。宾戈利亚菌含有重要的生物活性化合物，并可能具有抗氧化和抗肿瘤活性。