The purpose of this study was to develop a multi-layer diacetylene (PDA)-coated filter and to verify the proposed color response mechanism of PDA-coated filters to bacteria. Unlike other reports which immobilize PDA liposomes on solid support or create monolayer Langmuir films, multi-layer PDA-coated filters were generated by directly evaporating organic solvents. Different from incorporating functional headgroups or inserting phospholipids into PDA, we reported that bacterial growth can trigger the color change of PDA sensors without any modification or phospholipid insertion. The mechanism that pH change from bacteria metabolites lead to color change of PDA filter was proposed and verified by carefully designed dextrose-free medium with phenol red. Further, culturing Salmonella Typhimurium, E. coli, L. innocua and M. luteus with PDA-coated filters on phenol red agar in absence of dextrose verified the potential of applying PDA-coated filter for bacterial detection, specifically amine-producing bacteria. Thus, PDA-coated filters may be a useful tool for food safety and shelf life applications.

这项研究的目的是开发一种多层二乙炔（PDA）涂层滤光片，并验证PDA涂层滤光片对细菌的颜色响应机制。与其他将PDA脂质体固定在固体支持物上或形成单层Langmuir膜的报道不同，通过直接蒸发有机溶剂可生成多层PDA涂层滤膜。与并入功能性头基或将磷脂插入PDA中不同，我们报道细菌的生长可以触发PDA传感器的颜色变化，而无需进行任何修饰或插入磷脂。提出了由细菌代谢物引起的pH变化导致PDA过滤器颜色变化的机理，并通过精心设计的无葡萄糖酚红培养基进行了验证。此外，培养鼠伤寒沙门氏菌，ē。大肠杆菌，大号。英诺克和中号。藤黄微球菌在不存在葡萄糖的上酚红琼脂PDA-涂层滤波器验证施加PDA-涂覆的过滤器用于细菌检测，特别是胺生产菌的潜力。因此，涂有PDA的过滤器可能是食品安全和保质期应用的有用工具。