Due to the diffraction of light by gratings, use of optical filters is typically recommended in the emission exit in xenon lamp-based spectrofluorometer, especially for turbid samples. However, such diffraction also exists in the excitation, but is typically ignored. In this work, we demonstrated that the higher order diffraction of gratings in the excitation exit in the front of xenon lamp could cause real light, which could interference the fluorescence detection for NIR-emitting fluorophores. Besides, such interference light may also result in debated upconversion results in nowadays' nano-fluorophores. Overall, we wanted to call attention for the use of optical filters in the excitation exit to exclude potential biased results in fluorescence-related research, such as NIR-emissive fluorophores and long wavelength photocatalysis.

由于光栅会衍射光线，因此通常建议在基于氙灯的分光荧光计的发射出口使用光学滤光片，尤其是对于混浊的样品。然而，这种衍射也存在于激发中，但是通常被忽略。在这项工作中，我们证明了氙气灯前部激发出口处光栅的高阶衍射会引起真实的光，这可能会干扰发射NIR的荧光团的荧光检测。此外，这种干扰光还可能导致当今纳米荧光团的上转换结果争论不休。总的来说，我们希望引起人们的注意，在激发光出口使用光学滤光片，以排除与荧光相关的研究中潜在的偏倚结果，例如NIR发射荧光团和长波长光催化。