In this work, we described a green and simple approach for fabricating regenerated silk fibroin (RSF)/reduced graphene oxide (RGO) fibrous mats by reducing GO in the RSF/GO mats through hydrothermal treatment. As a comparison, RSF mat incorporated with graphene (Gr) was fabricated by solution mixing method. Results showed that the reduction of GO in the mats did take place during hydrothermal treatment. An increase in the crystallinity of the mats was induced by hydrothermal treatment. The maximum Young’s modulus of RSF/RGO mats reached (122.7 ± 3.1) MPa, which was at least 9-fold higher than RSF mats. As the mass ratio of RGO/RSF was 1.5/100, the average sheet resistance of RSF/RGO mats reached (1.2 ± 0.1)×10⁸ Ω/sq, which was better and more stable than that of RSF/Gr mats. Furthermore, cellular tests and antibacterial tests demonstrated that RSF/RGO mats possessed better biocompatibility and antibacterial property in comparison with RSF mats incorporated with GO or Gr. Thus, the RSF/RGO mats could be a good candidate for tissue engineering applications.

在这项工作中，我们描述了一种通过水热处理减少RSF / GO垫中的GO来制造再生丝素蛋白（RSF）/还原氧化石墨烯（RGO）纤维垫的绿色简单方法。作为比较，通过溶液混合法制造了结合有石墨烯（Gr）的RSF毡。结果表明，在水热处理期间确实发生了垫中GO的减少。通过水热处理引起垫的结晶度的增加。RSF / RGO垫的最大杨氏模量达到（122.7±3.1）MPa，比RSF垫高至少9倍。当RGO / RSF的质量比为1.5 / 100时，RSF / RGO垫的平均薄层电阻达到（1.2±0.1）×10 ⁸Ω/ sq，比RSF / Gr垫更好且更稳定。此外，细胞测试和抗菌测试表明，与掺有GO或Gr的RSF毡相比，RSF / RGO毡具有更好的生物相容性和抗菌性能。因此，RSF / RGO垫可能是组织工程应用的理想选择。