Background

In present study we investigated expression pattern of the special tissue markers.

SATB1 and PTEN to evaluate possible influence in pathophysiology and development of various biopsy proven kidney diseases.

Methods

The 32 kidney biopsy samples were analysed using light, immunofluorescence and electron microscopy. There were 19 samples in proliferative and 13 samples in non- proliferative group of renal diseases. As control group, 9 specimens of healthy kidney tissue taken after surgery of kidney tumour were used. SATB1 and PTEN markers were used for immunofluorescence staining. Analysed tissue structures were glomeruli, proximal convoluted tubules (PCT) and distal convoluted tubules (DCT). The number of SATB1 and PTEN cells were calculated and the data compared between kidney structures, disease groups and control specimens.

Results

Both markers were positive in all investigated kidney structures, with expression generally, more prominent in tubular epithelial cells than in glomeruli, with the highest staining intensity rate as well as highest rate of both markers in DCT of proliferative diseases group (SATB1 64.5 %, PTEN 52 %). There was statistically significant difference in SATB1 expression in all tissue structures of interest in proliferative as well as non- proliferative group compared to control group (p < 0.01-p < 0.0001). PTEN expression were found significantly decreased in PCT of both disease groups in regard to control (PTEN 25.3 % and 23.8 % vs. 41.1 % (p < 0.01 and p < 0.001 respectively).

Conclusion

SATB1 and PTEN could be considered as markers influenced in kidney disease development. SATB1/PTEN expression should be further investigated as useful markers of kidney disease activity as well as potential therapeutic target.

中文翻译：

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活检证实的肾脏疾病中的 SATB1 和 PTEN 表达模式

背景

在目前的研究中，我们调查了特殊组织标志物的表达模式。

SATB1 和 PTEN 评估对各种活检证实的肾脏疾病的病理生理学和发展的可能影响。

方法

使用光、免疫荧光和电子显微镜分析了 32 个肾活检样本。增殖组19例，非增殖组13例。以肾肿瘤手术后健康肾组织标本9例作为对照组。SATB1 和 PTEN 标记用于免疫荧光染色。分析的组织结构是肾小球、近曲小管 (PCT) 和远曲小管 (DCT)。计算SATB1和PTEN细胞的数量，并比较肾脏结构、疾病组和对照标本之间的数据。

结果

两种标志物在所有研究的肾脏结构中均呈阳性，表达一般在肾小管上皮细胞中比在肾小球中更显着，在增殖性疾病组的DCT中染色强度最高，两种标志物的染色率最高（SATB1 64.5 %，PTEN 52%）。与对照组相比，增殖和非增殖组中所有感兴趣的组织结构中的SATB1表达存在统计学显着差异（p  <  0.01-p < 0.0001）。发现与对照相比，两个疾病组的 PCT 中 PTEN 表达显着降低（PTEN 25.3 % 和 23.8 % 与 41.1 %（分别为 p < 0.01 和 p < 0.001）。

结论

SATB1 和 PTEN 可以被认为是影响肾脏疾病发展的标志物。应进一步研究 SATB1/PTEN 表达作为肾脏疾病活动的有用标志物以及潜在的治疗靶点。