Pumpkin ( Cucurbita maxima ) plants showing begomovirus-like yellowing, curling and stunting symptoms were observed in the Barka Governate, Oman in December 2016. Cloning and sequencing of rolling circle amplified circular DNA molecules showed the plants to be infected with watermelon chlorotic stunt virus (WmCSV); a bipartite Old World begomovirus that is a serious pathogen of cucurbit crops across the Middle East, Arabian Peninsula and North Africa. Although not known to interact with satellites, the risk of WmCSV possibly interacting with betasatellites and alphasatellites to agriculture in Oman, and beyond, was assessed. Tomato leaf curl betasatellite (ToLCB) and Ageratum enation alphasatellite (AEA) are commonly associated with other begomoviruses in Oman. A cognate pair of WmCSV DNA-A and DNA-B clones was highly infectious to Nicotiana benthamiana by Agrobacterium -mediated inoculation, inducing severe leaf curling and crumpling symptoms. Inoculation of only the DNA-A component with ToLCB or AEA did not result in symptoms or significant levels of the DNA-A or satellites in plants. Inoculation of both DNA-A and DNA-B with ToLCB or AEA resulted in a slight change in symptom phenotype and reduced DNA-A and DNA-B titers. Although ToLCB DNA levels in co-infected plants were low, the levels of AEA were high. This is the first identification of WmCSV infecting pumpkin in Oman and the isolates identified were typical of the virus previously identified in Oman, being distinct from the isolates occurring in the western Arabian Peninsula. Additionally the infectivity analyses suggest that WmCSV interaction with satellites, in contrast to some other bipartite begomoviruses, is unlikely to result in more serious disease in the field.

中文翻译：

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西瓜褪绿特技病毒与卫星的相互作用

2016 年 12 月在阿曼巴卡省观察到南瓜 (Cucurbita maxima) 植物表现出类似 begomovirus 的变黄、卷曲和发育迟缓症状。 滚环扩增环状 DNA 分子的克隆和测序显示该植物感染了西瓜褪绿矮化病毒（ WmCSV); 一种二分的旧世界 begomovirus，是中东、阿拉伯半岛和北非瓜类作物的严重病原体。虽然不知道与卫星相互作用，但评估了 WmCSV 可能与 β 卫星和 α 卫星相互作用对阿曼及其他地区农业的风险。番茄叶片卷曲 betasatellite (ToLCB) 和 Ageratum ation alphasatellite (AEA) 通常与阿曼的其他 begomoviruses 相关。一对同源的 WmCSV DNA-A 和 DNA-B 克隆通过农杆菌介导的接种对本氏烟草具有高度传染性，引起严重的卷叶和皱褶症状。仅用 ToLCB 或 AEA 接种 DNA-A 组分不会导致植物中出现症状或显着水平的 DNA-A 或卫星。用 ToLCB 或 AEA 接种 DNA-A 和 DNA-B 导致症状表型略有变化，并降低 DNA-A 和 DNA-B 滴度。尽管共感染植物中的 ToLCB DNA 水平较低，但 AEA 的水平较高。这是在阿曼首次发现 WmCSV 感染南瓜，所确定的分离株是先前在阿曼发现的病毒的典型特征，与发生在阿拉伯半岛西部的分离株不同。