Circular RNAs (circRNAs) are thought to be involved in the development of various malignancies. The expression and function of hsa_circ_0006916, a newly identified circRNA, in hepatocellular carcinoma remain unclear. Quantitative RT-PCR was used to detect hsa_circ_0006916 in hepatocellular carcinoma. In vitro function assays were conducted to explore growth and invasion of hepatocellular carcinoma cells. Next, the mechanism of hsa_circ_0006916 function in hepatocellular carcinoma was determined by luciferase reporter and RIP assays. Hsa_circ_0006916 was substantially overexpressed in hepatocellular carcinoma tissues and cells. High levels of hsa_circ_0006916 in hepatocellular carcinoma patients were associated with advanced clinical characteristics. Down-regulation of hsa_circ_0006916 decreased the growth and invasion of hepatocellular carcinoma cells in vitro. The results suggested that hsa_circ_0006916 acted as a sponge of miR-337-3p and had an important functional use in the regulation of STAT3 levels in hepatocellular carcinoma cells. Moreover, miR-337-3p inhibition or STAT3 overexpression abolished the effect of hsa_circ_0006916 suppression on the progression of hepatocellular carcinoma cells. Our data suggest a novel hsa_circ_0006916/miR-337-3p/STAT3 axis in hepatocellular carcinoma, and provide a new target for treatment.

中文翻译：

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hsa_circ_0006916 通过激活 miR-337-3p/STAT3 轴促进肝细胞癌进展

环状RNA（circRNA）被认为参与了各种恶性肿瘤的发展。hsa_circ_0006916 是一种新发现的 circRNA，在肝细胞癌中的表达和功能尚不清楚。定量RT-PCR用于检测肝细胞癌中的hsa_circ_0006916。进行体外功能测定以探索肝细胞癌细胞的生长和侵袭。接下来，hsa_circ_0006916 在肝细胞癌中的功能机制通过荧光素酶报告基因和 RIP 测定确定。Hsa_circ_0006916 在肝细胞癌组织和细胞中显着过表达。肝细胞癌患者中高水平的 hsa_circ_0006916 与晚期临床特征相关。hsa_circ_0006916 的下调降低了体外肝细胞癌细胞的生长和侵袭。结果表明 hsa_circ_0006916 充当 miR-337-3p 的海绵，在调节肝细胞癌细胞中 STAT3 水平方面具有重要的功能用途。此外，miR-337-3p 抑制或 STAT3 过表达消除了 hsa_circ_0006916 抑制对肝细胞癌细胞进展的影响。我们的数据表明肝细胞癌中存在新的 hsa_circ_0006916/miR-337-3p/STAT3 轴，并为治疗提供了新的靶点。miR-337-3p 抑制或 STAT3 过表达消除了 hsa_circ_0006916 抑制对肝细胞癌细胞进展的影响。我们的数据表明肝细胞癌中存在新的 hsa_circ_0006916/miR-337-3p/STAT3 轴，并为治疗提供了新的靶点。miR-337-3p 抑制或 STAT3 过表达消除了 hsa_circ_0006916 抑制对肝细胞癌细胞进展的影响。我们的数据表明肝细胞癌中存在新的 hsa_circ_0006916/miR-337-3p/STAT3 轴，并为治疗提供了新的靶点。