Three types of Co-xZr (x = 5, 7.5, and 10 wt.%) were treated with hydroxyapatite (HA) and used as an object to investigate the effect of HA coating on the surface and biocompatibility of Co-xZr alloys. And the protein adsorption and the subsequent biological behaviour of osteoblast, fibroblast and macrophages were also investigated. The surface microstructure and wettability were assessed by scanning electron microscopy (SEM) and static angle profilometer. To evaluate the biocompatibility of Co-xZr and Co-xZr-HA, we quantified plasma proteins adsorption by bicinchoninic acid assay (BCA), cytotoxicity and cell proliferation by cell counting kit-8 (CCK-8) and scanning electron microscopy (SEM). The results indicated that Co-xZr-HA alloy surfaces were more hydrophilic and had higher affinity to plasma proteins. Higher protein concentrations were found adsorbed onto Co-7.5Zr-HA and Co-10Zr-HA alloys. Cytotoxicity analysis indicated that HA coating improved the biocompatibility of Co-xZr alloys. Furthermore, the comparable results of co-incubation of Co-xZr-HA alloys with cells reveal cellular attachments to HA surfaces. HA was successfully formed on Co-xZr alloys and modified the surface structure and biocompatibility of the alloys. Co-10Zr-HA and Co-7.5Zr-HA had the most favourable properties and cytocompatibility, and therefore can be potentially used for dental implants.

用羟基磷灰石 (HA) 处理三种类型的 Co-xZr（x = 5、7.5 和 10 wt.%），并用作研究 HA 涂层对 Co-xZr 合金表面和生物相容性的影响。并且还研究了成骨细胞、成纤维细胞和巨噬细胞的蛋白质吸附和随后的生物学行为。通过扫描电子显微镜（SEM）和静态角度轮廓仪评估表面微观结构和润湿性。为了评估 Co-xZr 和 Co-xZr-HA 的生物相容性，我们通过二辛可宁酸测定法 (BCA) 量化血浆蛋白吸附，通过细胞计数试剂盒 8 (CCK-8) 和扫描电子显微镜 (SEM) 量化细胞毒性和细胞增殖. 结果表明，Co-xZr-HA合金表面更亲水，对血浆蛋白具有更高的亲和力。发现较高的蛋白质浓度吸附在 Co-7.5Zr-HA 和 Co-10Zr-HA 合金上。细胞毒性分析表明 HA 涂层提高了 Co-xZr 合金的生物相容性。此外，Co-xZr-HA 合金与细胞共孵育的类似结果揭示了细胞附着于 HA 表面。HA成功地在Co-xZr合金上形成并改变了合金的表面结构和生物相容性。Co-10Zr-HA和Co-7.5Zr-HA具有最有利的特性和细胞相容性，因此可以潜在地用于牙种植体。HA成功地在Co-xZr合金上形成并改变了合金的表面结构和生物相容性。Co-10Zr-HA和Co-7.5Zr-HA具有最有利的特性和细胞相容性，因此可以潜在地用于牙种植体。HA成功地在Co-xZr合金上形成并改变了合金的表面结构和生物相容性。Co-10Zr-HA和Co-7.5Zr-HA具有最有利的特性和细胞相容性，因此可以潜在地用于牙种植体。