ABSTRACT

For successful transplantation of Hematopoietic Stem cells (HSCs), it is quite necessary that efficient homing, engraftment and retention of HSC self-renewal capacity takes place, which is often restricted due to inadequate number of adult HSCs. Here, we report that short-term ex-vivo treatment of mouse bone marrow mononuclear cells (BMMNCs) to Sodium Hydrogen Sulfide (NaHS, hydrogen sulfide-H₂S donor) can be used as a possible strategy to overcome such hurdle. H₂S increases the expression of CXCR4 on HSPCs, enhancing their migration toward SDF-1α in-vitro and thus homing to BM niche. . Additionally, in-vitro studies revealed that H₂S has a role in activating mitochondria, thus, pushing quiescent HSCs into division. These results suggest a readily available and cost-effective method to facilitate efficient HSC transplantation.

摘要

为了成功移植造血干细胞（HSC），HSC的有效归巢、植入和保留自我更新能力是非常必要的，而这往往由于成体HSC数量不足而受到限制。在此，我们报告用硫化氢钠（NaHS，硫化氢-H ₂ S 供体）对小鼠骨髓单核细胞（BMMNC）进行短期离体治疗可作为克服这一障碍的可能策略。H ₂ S 增加 HSPC 上 CXCR4 的表达，增强它们在体外向 SDF-1α 的迁移，从而归巢到 BM 生态位。。此外，体外研究表明，H ₂ S 具有激活线粒体的作用，从而推动静止的 HSC 进入分裂。这些结果表明了一种容易获得且具有成本效益的方法来促进有效的 HSC 移植。