Temperature measurement at the nanoscale has brought insight to a wide array of research interests in modern chemistry, physics, and biology. These measurements have been enabled by the advent of nanothermometers, which relay nanoscale temperature information through the analysis of their intrinsic photophysical behavior. In the past decade, several nanothermometers have been developed including dyes, nanodiamonds, fluorescent proteins, nucleotides, and nanoparticles. However, temperature measurement using intact DNA has not yet been achieved. Here, we present a method to study the temperature sensitivity of the DNA molecule within a physiologic temperature range when complexed with fluorescent dye. We theoretically and experimentally report the temperature sensitivity of the DNA‐Hoechst 33342 complex in different sizes of double‐stranded oligonucleotides and plasmids, showing its potential use as a nanothermometer. These findings allow for extending the thermal study of DNA to several research fields including DNA nanotechnology, optical tweezers, and DNA nanoparticles.

中文翻译：

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利用 DNA 进行纳米测温

纳米尺度的温度测量为现代化学、物理学和生物学的广泛研究兴趣带来了洞察力。纳米温度计的出现使这些测量成为可能，纳米温度计通过分析其固有的光物理行为来传递纳米级温度信息。在过去的十年中，已经开发了几种纳米温度计，包括染料、纳米金刚石、荧光蛋白、核苷酸和纳米颗粒。然而，尚未实现使用完整 DNA 进行温度测量。在这里，我们提出了一种方法来研究 DNA 分子在与荧光染料复合时在生理温度范围内的温度敏感性。我们从理论上和实验上报告了 DNA-Hoechst 33342 复合物在不同大小的双链寡核苷酸和质粒中的温度敏感性，显示了其作为纳米温度计的潜在用途。这些发现允许将 DNA 的热研究扩展到几个研究领域，包括 DNA 纳米技术、光镊和 DNA 纳米颗粒。