• Phytochelatins (PCs) constitute an important mechanism for plants to resist heavy metal stress. Widely found in higher plants, they are small heavy metal binding peptides, synthesized through catalysis of phytochelatin synthase (PCS). We speculate that there may be PCS genes in Peacock grass (Tagetes patula L., Asteraceae), which is an important reason for its rich cadmium.
• In order to obtain the full‐length cDNA sequence of the PCS gene from T. patula L. used rapid amplification of cDNA ends (RACE). Meanwhile, Relative expression of TpPCS1 under different concentrations of cadmium (Cd) stress was analysed using quantitative real‐time polymerase chain reaction (qRT‐PCR).
• Results found ORF of TpPCS1 genes with a length of 1970 bp, a gene coding area length of 1764 bp, coding for 587 amino acids. Expression of TpPCS1 under Cd stress was tissue specific. TpPCS1 in the root showed higher expression, while expression in the leaf and seed was relatively low.
• This research demonstrates that expression of TpPCS1 enhanced the enrichment of cadmium in T. patula L. roots and could be used to construct a plant hyperexpression carrier that would provide new avenues for plant restoration technology.

中文翻译：

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age草PCS基因（TpPCS1）的克隆及镉胁迫下的表达分析

• 植物螯合素（PCs）是植物抵抗重金属胁迫的重要机制。它们是在高等植物中广泛发现的，是重金属结合的小肽，是通过催化植物螯合素合酶（PCS）合成的。我们推测孔雀草（Tagetes patula L.，Asteraceae）中可能存在PCS基因，这是其富含镉的重要原因。
• 为了从印度毛T（T。patula L.）获得PCS基因的全长cDNA序列，使用了cDNA末端（RACE）的快速扩增。同时，使用定量实时聚合酶链反应（qRT-PCR）分析了在不同浓度的镉（Cd）胁迫下TpPCS1的相对表达。
• 结果发现TpPCS1基因的ORF长度为1970 bp，基因编码区域长度为1764 bp，编码587个氨基酸。TpPCS1在Cd胁迫下的表达具有组织特异性。根中的TpPCS1表达较高，而叶和种子中的表达较低。
• 这项研究表明，TpPCS1的表达增强了T菜根中镉的富集，可用于构建植物超表达载体，为植物修复技术提供新途径。