Probiotics are nonpathogenic bacterial strains that exert beneficial effects on the host. Previous studies have shown that topical use of some strains of probiotic bacteria have good effects on the healing of cutaneous wounds. In the current study, the wound healing potentials of bacterial probiotics on diabetic cutaneous wounds were evaluated. The effects of probiotics on migration, the viability of fibroblasts, and macrophage proliferation were measured through using wound healing assay, methylthiazol tetrazolium assay, and bromodeoxyuridine, respectively. In this regard, in vivo diabetic wound healing experiments in Wistar rats following treatment with nontoxic concentrations of Lactobacillus bulgaricus and Lactobacillus plantarum were conducted. The histopathological and gene expression analyses were performed following removal of wound sites 3, 7, and 14 days postwounding. Results showed that treatment with probiotics accelerated the healing process of diabetic wounds and modulated the inflammatory cells in wound sites during a 14-day period postwounding. The altered mRNA levels of inflammatory cytokines were observed in wound sites following treatment with probiotics. The findings of the current study reveal that L. bulgaricus and L. plantarum could improve the healing of diabetic wounds via regulation of inflammation.

中文翻译：

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保加利亚乳杆菌和植物乳杆菌通过调节炎症因子促进糖尿病伤口愈合

益生菌是对宿主产生有益影响的非致病性细菌菌株。以往的研究表明，局部使用一些益生菌菌株对皮肤伤口的愈合有很好的效果。在目前的研究中，评估了细菌益生菌对糖尿病皮肤伤口的伤口愈合潜力。分别使用伤口愈合试验、甲基噻唑四唑试验和溴脱氧尿苷测定益生菌对迁移、成纤维细胞活力和巨噬细胞增殖的影响。在这方面，Wistar 大鼠在用无毒浓度的保加利亚乳杆菌和植物乳杆菌治疗后的体内糖尿病伤口愈合实验进行了。在伤后 3、7 和 14 天去除伤口部位后进行组织病理学和基因表达分析。结果表明，在伤后 14 天期间，益生菌治疗加速了糖尿病伤口的愈合过程并调节了伤口部位的炎症细胞。在用益生菌治疗后，在伤口部位观察到炎症细胞因子 mRNA 水平的改变。目前的研究结果表明，L . bulgaricus和L. plantarum可以通过调节炎症来改善糖尿病伤口的愈合。