Abstracts The present study was aimed to reveal the phytochemical composition and bio potentials of Bolbitis appendiculata (Willd.)K. Iwats. The qualitative and quantitative profiles viz., total phenolic, tannin, flavonoid, terpenoids, sterols content of B. appendiculata were determined. To reveal the bioactivity of B. appendiculata extracts, RBC Membrane stabilization heat induced haemolysis, In-vitro alpha-amylase inhibitory assay and Brine Shrimp lethality bioassay were performed. Among the tested extracts, acetone extracts of B. appendiculata illustrated high amount of phenol and triterpenoids than other studied extracts and methanolic extracts of B. appendiculata displayed maximum amount of tannins and sterols than other studied extracts. Highest amount of flavonoids was observed in chloroform extracts of B. appendiculata. All the studied extracts of B. appendiculata demonstrated dose dependent anti-inflammatory, anti-diabetic and toxicity activity. The anti-inflammatory activity of the B. appendiculata was as follows: petroleum ether > acetone > methanol > chloroform. The anti-diabetic properties of the B. appendiculata (25 µg/ mL) was as follows: acetone (56.12 %) > chloroform (37.96 %) > methanol (36.86 %) > petroleum ether (34.56 %). The toxicity of the B. appendiculata was as follows:petroleum ether > chloroform > methanol > acetone.The observed results of B. appendiculata extracts suggested that B. appendiculata can be considered as an alternative source for plant-derived anti-inflammatory and anti-diabetic agents. Further studies may bring out the active principles with anti-inflammatory and anti-diabetic potentials.

中文翻译：

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Bolbitis appendiculata (Willd.) K. Iwats 的植物化学特征和生物活性。提取物

摘要 本研究旨在揭示 Bolbitis appendiculata (Willd.)K 的植物化学成分和生物潜力。艾瓦茨。定性和定量特征，即 B. appendiculata 的总酚、单宁、类黄酮、萜类、甾醇含量被确定。为了揭示 B. appendiculata 提取物的生物活性，进行了红细胞膜稳定化热诱导溶血、体外 α-淀粉酶抑制试验和盐水虾致死率生物试验。在测试的提取物中，B. appendiculata 的丙酮提取物比其他研究的提取物显示出大量的苯酚和三萜类化合物，而 B. appendiculata 的甲醇提取物比其他研究的提取物显示出最大量的单宁和甾醇。在 B. appendiculata 的氯仿提取物中观察到最高含量的类黄酮。所有研究的 B. appendiculata 提取物均表现出剂量依赖性抗炎、抗糖尿病和毒性活性。B. appendiculata 的抗炎活性如下：石油醚>丙酮>甲醇>氯仿。B. appendiculata (25 µg/mL) 的抗糖尿病特性如下：丙酮 (56.12 %) > 氯仿 (37.96 %) > 甲醇 (36.86 %) > 石油醚 (34.56 %)。B. appendiculata 的毒性为：石油醚>氯仿>甲醇>丙酮。 B. appendiculata 提取物的观察结果表明，B. appendiculata 可作为植物源性抗炎和抗炎的替代来源。糖尿病药剂。进一步的研究可能会发现具有抗炎和抗糖尿病潜力的活性成分。appendiculata 表现出剂量依赖性的抗炎、抗糖尿病和毒性活性。B. appendiculata 的抗炎活性如下：石油醚>丙酮>甲醇>氯仿。B. appendiculata (25 µg/mL) 的抗糖尿病特性如下：丙酮 (56.12 %) > 氯仿 (37.96 %) > 甲醇 (36.86 %) > 石油醚 (34.56 %)。B. appendiculata 的毒性为：石油醚>氯仿>甲醇>丙酮。 B. appendiculata 提取物的观察结果表明，B. appendiculata 可作为植物源性抗炎和抗炎的替代来源。糖尿病药剂。进一步的研究可能会发现具有抗炎和抗糖尿病潜力的活性成分。appendiculata 表现出剂量依赖性的抗炎、抗糖尿病和毒性活性。B. appendiculata 的抗炎活性如下：石油醚>丙酮>甲醇>氯仿。B. appendiculata (25 µg/mL) 的抗糖尿病特性如下：丙酮 (56.12 %) > 氯仿 (37.96 %) > 甲醇 (36.86 %) > 石油醚 (34.56 %)。B. appendiculata 的毒性为：石油醚>氯仿>甲醇>丙酮。 B. appendiculata 提取物的观察结果表明，B. appendiculata 可作为植物源性抗炎和抗炎的替代来源。糖尿病药剂。进一步的研究可能会发现具有抗炎和抗糖尿病潜力的活性成分。