Disease-modifying osteoarthritis (OA) therapy is not yet available. Several adjuvant therapies have demonstrated promising results in the treatment of OA. The present study aimed to investigate the therapeutic effects and underlying mechanisms of a combination of Lactobacillus acidophilus, vitamin B, and curcumin in the treatment of OA.

Monosodium iodoacetate (MIA)-induced arthritis of the knee joint in rat was used as an animal model of human OA. The combination of L. acidophilus LA-1, vitamin B, and curcumin or a saline solution was given orally. Pain was measured according to the paw withdrawal latency, and paw withdrawal threshold.

Cartilage destruction was analyzed using histomorphological techniques and the Mankin scoring system. Protein expression in the joint was examined using immunohistochemistry. The effects of the combination of L. acidophilus LA-1, vitamin B, and curcumin on mRNA levels in chondrocytes stimulated with interleukin (IL)-1β were analyzed using real-time polymerase chain reaction.

The combination of L. acidophilus, vitamin B, and curcumin effectively downregulated Th17 cells and the related cytokine IL-17, thereby maintained the Treg population, and increased the expression of the Treg-related cytokine IL-10 in human peripheral blood mononuclear cells. The OA animal model exhibited reduced pain and preservation of cartilage in response to the combination treatment. The expression levels of pro-inflammatory cytokines and the catabolic, matrix metalloproteinase-13 (MMP-13), were decreased, whereas the expression of the anabolic tissue inhibitors of metalloproteinases (TIMPs) were upregulated in response to the drug combination.

The combination of L. acidophilus, vitamin B, and curcumin was beneficial in OA treatment, controlling the inflammatory response via regulation of the Th17/Treg population and reducing the expression of pro-inflammatory cytokines in human peripheral blood mononuclear cells. The combination treatment also preserved cartilage, suppressed osteoclastogenesis, and regulated the anabolic/catabolic imbalance. These findings indicate the therapeutic potential of combination use of L. acidophilus, vitamin B, and curcumin in patients with OA.

目前尚无改善疾病的骨关节炎 (OA) 疗法。几种辅助疗法在 OA 的治疗中显示出有希望的结果。本研究旨在探讨嗜酸乳杆菌、维生素 B 和姜黄素联合治疗 OA的治疗效果和潜在机制。

碘乙酸钠 (MIA) 诱导的大鼠膝关节关节炎被用作人类 OA 的动物模型。的组合的嗜酸乳杆菌LA-1，维生素B和姜黄素或盐溶液经口给药。根据缩爪潜伏期和缩爪阈值测量疼痛。

使用组织形态学技术和 Mankin 评分系统分析软骨破坏。使用免疫组织化学检查关节中的蛋白质表达。使用实时聚合酶链反应分析嗜酸乳杆菌 LA-1、维生素 B 和姜黄素的组合对白介素 (IL)-1β 刺激的软骨细胞中 mRNA 水平的影响。

的组合的嗜酸乳杆菌，维生素B和姜黄素有效下调Th17细胞和相关的细胞因子IL-17，从而保持了调节性T细胞群，而且增加了在人外周血单核细胞的调节性T细胞相关的细胞因子IL-10的表达。OA 动物模型响应联合治疗表现出减轻的疼痛和软骨的保护。促炎细胞因子和分解代谢的基质金属蛋白酶-13 (MMP-13) 的表达水平降低，而金属蛋白酶合成代谢组织抑制剂 (TIMP) 的表达水平响应药物组合而上调。

嗜酸乳杆菌、维生素 B 和姜黄素的组合有利于 OA 治疗，通过调节 Th17/Treg 群控制炎症反应并减少人外周血单核细胞中促炎细胞因子的表达。联合治疗还保留了软骨，抑制了破骨细胞生成，并调节了合成代谢/分解代谢失衡。这些发现表明联合使用嗜酸乳杆菌、维生素 B 和姜黄素对 OA 患者具有治疗潜力。