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CRISPR_Cas systems for fungal research
Fungal Biology Reviews ( IF 6 ) Pub Date : 2020-11-02 , DOI: 10.1016/j.fbr.2020.10.002
Jean-Paul Ouedraogo , Adrian Tsang

Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have improved our ability to precisely edit genomes and control gene expression. We summarize here the knowledge gained from using CRISPR-Cas9 and CRISPR-Cas12a in fungal research. Also discussed are strategies developed for limiting the occurrences of off-target mutations caused by CRISPR-Cas genome editing.



中文翻译:

用于真菌研究的CRISPR_Cas系统

使用Cas9和Cas12a核酸内切酶的基因组编辑CRISPR-Cas系统提高了我们精确编辑基因组和控制基因表达的能力。我们在这里总结在真菌研究中使用CRISPR-Cas9和CRISPR-Cas12a所获得的知识。还讨论了为限制由CRISPR-Cas基因组编辑引起的脱靶突变的发生而开发的策略。

更新日期:2020-11-16
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