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Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) alleviate water deficit effects in soybean: from gene expression up to growth performance
Environmental and Experimental Botany ( IF 5.7 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.envexpbot.2020.104303 Inae Braga-Reis , Daniel Moreira Neris , Alessandra Ferreira Ribas , Luiz Gonzaga Esteves Vieira , Gustavo Maia Souza
Environmental and Experimental Botany ( IF 5.7 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.envexpbot.2020.104303 Inae Braga-Reis , Daniel Moreira Neris , Alessandra Ferreira Ribas , Luiz Gonzaga Esteves Vieira , Gustavo Maia Souza
Abstract Bioregulators act as priming agents under abiotic stress conditions. Bioregulators such as gamma-aminobutyric acid (GABA) and acetylcholine (ACh) affect the efficiency of the antioxidant system and the regulation of the stomatal aperture, respectively. The aim of this study was to verify a possible synergistic effect among these bioregulators as attenuators of the effects of water deficiency in Glycine max. We combined the application of GABA and ACh at 2.0 mM in soybean plants under different water regimes. The factors studied were: 1) application of Gaba and ACh in seeds (S); in leaves (L); in seeds and leaves (SL); control without application (C); and (2) water regimes at 100% field capacity (FC) and water deficit (WD). When the application of bioregulators were removed from the process, a severe decrease in photosynthesis capacity (93%) was observed on the sixth day (after withholding water), as well as a higher expression of the genes known to be induced by water deficit. The combination of GABA and ACh applied to seeds and leaves under water deficit resulted in a lower decline in photosynthesis, as well as better water-use efficiency and biomass production. Soybean plants subjected to this treatment also showed lower expression of GmABA2, GmLEA3 and GmP5CS genes, lower proline content and increased activity of SOD, CAT and APX compared to the control treatment. The results indicate that the combined exogenous application of GABA and ACh in soybean plants acted to promote increased tolerance to water deficit, showing their potential for use on agricultural areas which are prone to droughts.
中文翻译:
γ-氨基丁酸 (GABA) 和乙酰胆碱 (ACh) 缓解大豆缺水效应:从基因表达到生长性能
摘要 生物调节剂在非生物胁迫条件下充当启动剂。γ-氨基丁酸 (GABA) 和乙酰胆碱 (ACh) 等生物调节剂分别影响抗氧化系统的效率和气孔孔径的调节。本研究的目的是验证这些生物调节剂之间可能的协同作用,作为甘氨酸最大缺水影响的减弱剂。我们将 GABA 和 ACh 以 2.0 mM 的浓度应用于不同水分条件下的大豆植物。研究的因素是:1) 在种子 (S) 中施用 Gaba 和 ACh;在叶子 (L); 在种子和叶子中(SL);无需申请即可控制 (C);(2) 100% 田间持水量 (FC) 和缺水 (WD) 的水情。当从过程中去除生物调节剂的应用时,在第六天(停水后)观察到光合作用能力严重下降(93%),以及已知由缺水诱导的基因表达更高。GABA 和 ACh 的组合应用于缺水的种子和叶子,导致光合作用下降较少,以及更好的水分利用效率和生物量生产。与对照处理相比,接受该处理的大豆植物还表现出 GmABA2、GmLEA3 和 GmP5CS 基因的较低表达、较低的脯氨酸含量和增加的 SOD、CAT 和 APX 活性。结果表明,在大豆植物中外源性施用 GABA 和 ACh 可提高对缺水的耐受性,显示出它们在易受干旱影响的农业地区使用的潜力。
更新日期:2021-02-01
中文翻译:
γ-氨基丁酸 (GABA) 和乙酰胆碱 (ACh) 缓解大豆缺水效应:从基因表达到生长性能
摘要 生物调节剂在非生物胁迫条件下充当启动剂。γ-氨基丁酸 (GABA) 和乙酰胆碱 (ACh) 等生物调节剂分别影响抗氧化系统的效率和气孔孔径的调节。本研究的目的是验证这些生物调节剂之间可能的协同作用,作为甘氨酸最大缺水影响的减弱剂。我们将 GABA 和 ACh 以 2.0 mM 的浓度应用于不同水分条件下的大豆植物。研究的因素是:1) 在种子 (S) 中施用 Gaba 和 ACh;在叶子 (L); 在种子和叶子中(SL);无需申请即可控制 (C);(2) 100% 田间持水量 (FC) 和缺水 (WD) 的水情。当从过程中去除生物调节剂的应用时,在第六天(停水后)观察到光合作用能力严重下降(93%),以及已知由缺水诱导的基因表达更高。GABA 和 ACh 的组合应用于缺水的种子和叶子,导致光合作用下降较少,以及更好的水分利用效率和生物量生产。与对照处理相比,接受该处理的大豆植物还表现出 GmABA2、GmLEA3 和 GmP5CS 基因的较低表达、较低的脯氨酸含量和增加的 SOD、CAT 和 APX 活性。结果表明,在大豆植物中外源性施用 GABA 和 ACh 可提高对缺水的耐受性,显示出它们在易受干旱影响的农业地区使用的潜力。