Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-11-02 , DOI: 10.1016/j.colsurfb.2020.111445 Archana Mishra 1 , Vipul K Pandey 2 , Bhavani S Shankar 3 , Jose S Melo 1
Biohybrids (a combination of biological material and inorganic nanoparticles) offer a number of advantages like improved functionality over conventional materials.Thus, to understand the practical application of biohybrids as drug carriers, a biohybrid drug carrier of colloidal silica nanoparticles (NP)-sodium alginate loaded with doxorubicin (Dox-biohybrid) was synthesized by evaporation induced self-assembly (EISA) using spray drying technique. Further, the morphology, size and interactions between various components of the biohybrid were studied through SEM, DLS and FTIR techniques. The drug loading efficiency, release profile, cellular uptake and cytotoxicity of Dox-biohybrid was investigated and compared with free Dox. The drug loading efficiencies of Dox-biohybrid, Dox-silica NP and Dox-sodium alginate were 93.7 %, 96.4 % and 88.3 % respectively. In vitro release study showed a slow release of entrapped Dox from Dox-biohybrid as compared to other carriers. This release was also pH-responsive with significantly higher cumulative drug release at pH 5.5 (cancer microenvironment) in comparison to pH 7.4 (physiological conditions). The empty biohybrid carrier did not show cytotoxicity to normal mouse lymphocytes upto a concentration of 25 μg/mL which was used further. The uptake of Dox from Dox-biohybrid by A549 cells was more than 2fold as compared to uptake from free Dox. in vitro viability assay revealed that treatment of lung carcinoma A549 cells with Dox-biohybrid resulted in 50 % loss of cell viability at 500 nM, compared to only 12 % loss with free Dox. Thus, we report the synthesis of a novel biohybrid drug delivery system by means of spray drying process that has promising applications in cancer treatment.
中文翻译:
喷雾干燥是合成二氧化硅纳米颗粒-海藻酸钠生物杂化药物载体的有效途径
生物混合物(生物材料和无机纳米颗粒的组合)具有许多优点,例如比常规材料具有更好的功能性。采用喷雾干燥技术,通过蒸发诱导自组装(EISA)合成了负载有阿霉素(Dox-biohybrid)的药物。此外,通过SEM,DLS和FTIR技术研究了生物杂交体的形态,大小和相互作用。研究了Dox生物杂合体的载药效率,释放曲线,细胞摄取和细胞毒性,并将其与游离Dox进行了比较。Dox-生物杂种,Dox-二氧化硅NP和Dox-海藻酸钠的载药率分别为93.7%,96.4%和88。体外释放研究表明,与其他载体相比,捕获的Dox从Dox生物杂种中的释放缓慢。与pH 7.4(生理条件)相比,该释放也是pH响应的,在pH 5.5(癌症微环境)下累积药物释放明显更高。空的生物杂交载体在浓度高达25μg/ mL时对正常小鼠淋巴细胞没有细胞毒性,可进一步使用。与来自游离Dox的摄取相比,A549细胞从Dox-生物杂种中摄取Dox的程度是其两倍。体外生存力测定表明,用Dox生物杂交处理肺癌A549细胞在500 nM时导致50%的细胞生存力丧失,而游离Dox仅损失12%。因此,我们报告了通过喷雾干燥过程合成的新型生物混合药物递送系统,该方法在癌症治疗中具有广阔的应用前景。