In this work, a series of novel piperazine-2,5-dione derivatives bearing indole analogs (2a-2q) was designed and synthesized. The synthesized compounds were characterized by IR, ¹H NMR, ¹³C NMR spectroscopy, and ESI-MS. They were then evaluated for their anti-depressant, anti-inflammatory, and analgesic activities in vivo. The experimental results revealed that all the compounds showed clear anti-depressant, anti-inflammatory, and analgesic effects at a dose of 10 mg/kg. Among them, compounds 2e and 2q exhibited the best anti-depressant effects (the percent decreases in the duration of immobility were 70.2% and 71.2%, respectively), which were similar to that of fluoxetine (67.9%) in the forced swim test. Additionally, compounds 2e and 2q also displayed good anti-inflammatory and analgesic activities. Literature reports have highlighted the anti-inflammatory and analgesic effects of anti-depressant drugs, suggesting that they may have a similar mechanism of action. Therefore, further studies to investigate the possible mechanisms of action of compounds 2e and 2q are warranted.

在这项工作中，设计并合成了一系列带有吲哚类似物（2a-2q）的新型哌嗪-2,5-二酮衍生物。通过IR，¹ H NMR，¹³ C NMR光谱和ESI-MS对合成的化合物进行表征。然后评估它们的体内抗抑郁，抗炎和镇痛活性。实验结果表明，所有化合物在10 mg / kg的剂量下均显示出明显的抗抑郁，抗炎和镇痛作用。其中，化合物2e和2q表现出最好的抗抑郁作用（固定时间减少的百分比分别为70.2％和71.2％），与强迫游泳测试中的氟西汀（67.9％）相似。另外，化合物2e和2q还显示出良好的抗炎和止痛活性。文献报告强调了抗抑郁药的抗炎和镇痛作用，表明它们可能具有相似的作用机理。因此，有必要进一步研究化合物2e和2q的可能作用机理。