Electropermeabilization of the cell membrane is a technique used to facilitate penetration of impermeant molecules into cells. Although there are studies regarding the mechanism of processes occurring after electropermeabilization, the relationship between electropermeabilization and associated phenomena (e.g. generation of reactive oxygen species, endocytosis, lipid peroxidation, etc.) is yet to be elucidated. This work aimed to get information on the changes in the packing of the bilayer lipids and their peroxidation induced by application of electroporation pulses. We used a specially designed system of electrodes which allowed performing electropermeabilization of cells in suspension simultaneously with time-dependent measurements of fluorescence and temperature. The kinetics of membrane packing and production of reactive oxygen species were studied using various conductivity buffers (0.01, 0.04 and 0.14 S/m) and different number of 1 kV/cm bipolar pulses (1–50). Two categories of effects were observed: a thermal effect, consisting in an increased bilayer disorder (a deeper penetration of water into the hydrophobic core), and a nonthermal effect, leading to a higher degree of lipids packing, the latter being attributed to a peroxidation process. An analysis of the permeabilization conditions in which one of these two processes predominates was performed.

细胞膜的电透化是用于促进不渗透分子渗透进入细胞的技术。尽管有关于电渗透后过程发生机理的研究，但电渗透与相关现象（例如活性氧的产生，内吞作用，脂质过氧化作用等）之间的关系尚待阐明。这项工作旨在获得有关双层脂质堆积和电穿孔脉冲诱导过氧化作用的信息。我们使用了专门设计的电极系统，该系统允许对悬浮液中的细胞进行电透化，同时进行荧光和温度的时间依赖性测量。使用各种电导率缓冲液（0.01、0.04和0.14 S / m）和不同数量的1 kV / cm双极脉冲（1至50）研究了膜填充和活性氧生成的动力学。观察到两种作用：一种是热作用，包括增加的双层紊乱（水更深地渗透到疏水核中）；另一种是非热作用，导致脂质堆积程度更高，后者归因于过氧化作用处理。对这两个过程之一占主导地位的透化条件进行了分析。其表现为双层紊乱增加（水更深地渗透到疏水核中）和非热效应，导致脂质堆积程度更高，后者归因于过氧化过程。对这两个过程之一占主导地位的透化条件进行了分析。表现为双层紊乱增加（水更深地渗透到疏水核中）和非热效应，导致脂质堆积程度更高，后者归因于过氧化过程。对这两个过程之一占主导地位的透化条件进行了分析。