This study was conducted to determine optimum image capture frame rates (FRO) when there was evaluation of different types of counting chambers used for CASA-Mot determinations of stallion sperm motility. Sperm VCL was determined at frame rates of 25–250 f/s in: 1) Spermtrack® (Spk) 10 and 20 chambers (drop displacement-type chambers 10 and 20 μm-deep respectively; and 2) ISAS®D4C10, ISAS®D4C20 (10 and 20 μm-deep respectively) and ISAS®D4C20 L (20 μm-deep) capillary loaded chambers. Values for different sperm kinematic variables were determined using each chamber at 250 f/s, which is the maximum frame rate that the software can be used for analyses. With evaluation of Spk chambers, there was a greater curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) values (P < 0.05) than with capillary loaded chambers, with there being greatest values with 20 μm-deep chambers. With the Spk10 chamber, VCL and ALH were greater at the chamber centre than periphery. There were no such differences for the Spk20 chamber. With evaluation of the D4C10 chamber, VSL and STR were less when there was a sperm deposition point towards the chamber end, while there were the opposite for the D4C20 chamber. When there was evaluation of the D4C20 chamber, there were also greater VCL, WOB and BCF values in distal areas. With use of most of these chambers, data should be collected from different fields and means determined, however, this is not necessary with Spk20 chambers.

进行这项研究是为了在评估用于 CASA-Mot 确定种马精子活力的不同类型的计数室时，确定最佳图像捕获帧速率 (FRO)。精子 VCL 在 25–250 f/s 的帧速率下测定：1) Spermtrack® (Spk) 10 和 20 个腔室（分别为 10 和 20 μm 深的液滴置换型腔室；和 2）ISAS®D4C10、ISAS® D4C20（分别为 10 和 20 μm 深）和 ISAS®D4C20 L（20 μm 深）毛细管加载室。使用每个腔室以 250 f/s 确定不同精子运动学变量的值，这是该软件可用于分析的最大帧速率。通过对 Spk 腔室的评估，有更大的曲线速度 (VCL)、平均路径速度 (VAP)、直线速度 (STR)、P  < 0.05) 与毛细管加载腔室相比，20 μm 深腔室的值最大。对于 Spk10 腔室，腔室中心的 VCL 和 ALH 大于外围。Spk20 室没有这种差异。通过对 D4C10 腔室的评估，当精子沉积点朝向腔室末端时，VSL 和 STR 较小，而 D4C20 腔室则相反。在评估 D4C20 腔室时，远端区域的 VCL、WOB 和 BCF 值也更高。使用这些腔室中的大多数时，应从不同的领域收集数据并确定方法，但是，这对于 Spk20 腔室来说不是必需的。