The present study focuses on the development and validation of a high-performance thin-layer chromatography (HPTLC)‒densitometry method for the identification and quantification of resveratrol in the 70% methanolic extract of Morus alba fruits. The separation was achieved with the developer hexane‒ethyl acetate‒glacial acetic acid (4:6:0.1, V/V) on TLC aluminum plate pre-coated with silica gel 60 F₂₅₄. Densitometric scanning was carried out at a wavelength of 302 nm in absorbance mode. The peak corresponding to reference standard resveratrol and that of the extract had the same R_F of 0.556. The method was validated by following the International Conference of Harmonization (ICH) guidelines for precision, accuracy and reproducibility. The regression equation showed a polynomial function with a correlation coefficient of 0.994 ± 4.20% (R ± SD). The limit of detection and limit of quantification were 16 and 45 ng/band, respectively. The content of resveratrol was found to be 31.94 μg/100 mg of 70% methanolic extract of M. alba. The validated HPTLC method can be routinely used for the quality control and standardization of the bioactive marker resveratrol and formulations containing M. alba.

本研究的重点是开发和验证一种高效薄层色谱法（HPTLC）的光密度法，用于鉴定和定量桑树果实70％甲醇提取物中的白藜芦醇。用在预涂有硅胶60 F _254的TLC铝板上的显影剂己烷‒乙酸乙酯‒冰醋酸（4：6：0.1，  V / V）进行分离。以吸收模式在302nm的波长下进行光密度扫描。对应于参考标准白藜芦醇和该提取物的峰具有相同的- [R _˚F为0.556。该方法已通过遵循国际协调会议（ICH）的精密度，准确性和可重复性指南进行了验证。回归方程显示多项式函数，相关系数为0.994±4.20％（R  ±  SD）。检测限和定量限分别为16和45 ng / band。发现白藜芦醇的含量为31.94μg/ 100mg的白念珠菌的甲醇提取物/ 100mg 。经过验证的HPTLC方法可常规用于生物活性标记白藜芦醇和含白念珠菌制剂的质量控制和标准化。